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Open Access Green as soon as Postprint is submitted to ZB.
In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography.
Nat. Methods 5, 45-47 (2008)
We report a technique for fluorescence tomography that operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The method uses multi-projection illumination and photon transport description in opaque tissues. We demonstrate whole-body three-dimensional visualization of the morphogenesis of GFP-expressing salivary glands and wing imaginal discs in living Drosophila melanogaster pupae in vivo and over time.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
1548-7091
e-ISSN
1548-7105
Journal
Nature Methods
Quellenangaben
Volume: 5,
Issue: 1,
Pages: 45-47
Publisher
Nature Publishing Group
Publishing Place
New York, NY
Reviewing status
Peer reviewed
Institute(s)
Institute of Biological and Medical Imaging (IBMI)