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Vinegoni, C.* ; Pitsouli, C.* ; Razansky, D. ; Perrimon, N.* ; Ntziachristos, V.

In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography.

Nat. Methods 5, 45-47 (2008)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
We report a technique for fluorescence tomography that operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The method uses multi-projection illumination and photon transport description in opaque tissues. We demonstrate whole-body three-dimensional visualization of the morphogenesis of GFP-expressing salivary glands and wing imaginal discs in living Drosophila melanogaster pupae in vivo and over time.
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Publication type Article: Journal article
Document type Scientific Article
Language english
Publication Year 2008
HGF-reported in Year 0
ISSN (print) / ISBN 1548-7091
e-ISSN 1548-7105
Journal Nature Methods
Quellenangaben Volume: 5, Issue: 1, Pages: 45-47 Article Number: , Supplement: ,
Publisher Nature Publishing Group
Publishing Place New York, NY
Reviewing status Peer reviewed
POF-Topic(s) 30205 - Bioengineering and Digital Health
Research field(s) Enabling and Novel Technologies
PSP Element(s) G-505500-001
Erfassungsdatum 2008-09-10