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Kornblum, C.* ; Nicholls, T.J.* ; Haack, T.B. ; Schöler, S.* ; Peeva, V.* ; Danhauser, K. ; Hallmann, K.* ; Zsurka, G.* ; Rorbach, J.* ; Iuso, A. ; Wieland, T. ; Sciacco, M.* ; Ronchi, D.* ; Comi, G.P.* ; Moggio, M.* ; Quinzii, C.M.* ; DiMauro, S.* ; Calvo, S.E.* ; Mootha, V.K.* ; Klopstock, T.* ; Strom, T.M. ; Meitinger, T. ; Minczuk, M.* ; Kunz, W.S.* ; Prokisch, H.

Loss-of-function mutations in MGME1 impair mtDNA replication and cause multisystemic mitochondrial disease.

Nat. Genet. 45, 214-219 (2013)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Known disease mechanisms in mitochondria! DNA (mtDNA) maintenance disorders alter either the mitochondrial replication machinery (POLG, POLG2 and C10orf2)(1-3) or the biosynthesis pathways of deoxyribonucleoside 5'-triphosphates for mtDNA synthesis(4-11). However, in many of these disorders, the underlying genetic defect has yet to be discovered. Here, we identify homozygous nonsense and missense mutations in the orphan gene C20orf72 in three families with a mitochondrial syndrome characterized by external ophthalmoplegia, emaciation and respiratory failure. Muscle biopsies showed mtDNA depletion and multiple mtDNA deletions. C20orf72, hereafter MGME1 (mitochondrial genome maintenance exonuclease 1), encodes a mitochondria! RecB-type exonuclease belonging to the PD-(D/E)XK nuclease superfamily. We show that MGME1 cleaves single-stranded DNA and processes DNA flap substrates. Fibroblasts from affected individuals do not repopulate after chemically induced mtDNA depletion. They also accumulate intermediates of stalled replication and show increased levels of 7S DNA, as do MGME1-depleted cells. Thus, we show that MGME1-mediated mtDNA processing is essential for mitochondrial genome maintenance.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Progressive External Ophthalmoplegia ; Base Excision-repair ; Dna Depletion ; Protein ; Deficiency ; Gene ; Polg ; Maintenance ; Deletions ; Twinkle
ISSN (print) / ISBN 1061-4036
e-ISSN 1546-1718
Journal Nature Genetics
Quellenangaben Volume: 45, Issue: 2, Pages: 214-219 Article Number: , Supplement: ,
Publisher Nature Publishing Group
Publishing Place New York, NY
Reviewing status Peer reviewed
Institute(s) Institute of Human Genetics (IHG)