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Harlaar, N.J. ; Kelder, W. ; Sarantopoulos, A. ; Bart, J.* ; Themelis, G. ; van Dam, G.M.* ; Ntziachristos, V.

Real-time Near Infrared Fluorescence (NIRF) intra-operative imaging in ovarian cancer using an αvβ3-integrin targeted agent.

Gynecol. Oncol. 128, 590-595 (2013)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
BACKGROUND: In ovarian cancer, optimal cytoreductive surgery is of the utmost importance for long-term survival. The ability to visualize minuscule tumor deposits is important to ensure complete resection of the tumor. The purpose of our study was to estimate the in vivo sensitivity, specificity and diagnostic accuracy of an intra-operative fluorescence imaging system combined with an α(v)β(3)-integrin targeted near-infrared fluorescent probe. METHOD: Tumor bearing mice were injected intravenously with a fluorescent probe targeting α(v)β(3) integrins. Fluorescent spots and non-fluorescent tissue were identified and resected. Standard histopathology and fluorescence microscopy were used as gold-standard for tumor detection. RESULTS: Fifty-eight samples excised with support of intra-operative image-guided surgery were analyzed. The mean target to background ratio was 2.2 (SD 0.5). The calculated sensitivity of the imaging system was 95%, and the specificity was 88% with a diagnostic accuracy of 96.5%. CONCLUSION: Near-infrared image-guided surgery in this model has a high diagnostic accuracy and a fair target to background ratio that supports the development towards clinical translation of α(v)β(3)-integrin targeted imaging.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Ovarian Cancer ; Intraoperative Fluorescence Imaging ; Integrins; In-vivo Fluorescence ; Breast-cancer ; Model ; Identification ; Metastases ; Integrins ; Surgery
ISSN (print) / ISBN 0090-8258
e-ISSN 1095-6859
Journal Gynecologic Oncology
Quellenangaben Volume: 128, Issue: 3, Pages: 590-595 Article Number: , Supplement: ,
Publisher Elsevier
Reviewing status Peer reviewed
Institute(s) Institute of Biological and Medical Imaging (IBMI)