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Bacterial community structure and colonization patterns of Fagus sylvatica L. ectomycorrhizospheres as determined by fluorescence in situ hybridization and confocal laser scanning microscopy.
Mycorrhiza 9, 271-278 (2000)
The bacterial community structure of ecto-mycorrhizospheres on beech (Fagus sylvatica) grown in natural forest soil in southern Germany was examined by fluorescence in situ hybridization (FISH) using fluorescent oligonucleotide probes, targeting phylogenetic relevant sequences of the 16S and 23S rRNA. Lactarius subdulcis, L. vellereus, L. rubrocinctus and Laccaria amethystina were found to be the prevalent fungi forming ectomycorrhizae with F. sylvatica. For FISH studies using confocal laser scanning microscopy, oligonucleotide probes labeled with carboxymethylindocyanine-succinimidyl ester allowed detection of associated bacteria, because the autofluorescence of ectomycorrhiza samples could be overcome in the infrared. Bacteria of the alpha-, beta and gamma-subclasses of the proteobacteria were detected in high numbers on mantle surfaces, while members of other phylogenetically defined groups were found in smaller numbers. This contrasts with previous published results on the cultivation of mycorrhiza-associated bacteria. Hybridizing bacteria were also found within damaged cells of the hyphal mantle of L. rubrocinctus, as well as on emanating hyphae of L. amethystina. Using a newly developed extraction protocol for bacteria associated with ectomycorhizas, the two most common fungi on F. sylvatica, L. vellereus and L. subdulcis, were mostly associated with members of the alpha- and beta-subclasses of the proteobacteria. The proportion of hybridizing bacteria varied between the two ectomycorrhizae, which were thus host to distinct populations of bacteria.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
beech; ectomycorrhizae; FISH; CLSM; bacterial community structure; TARGETED OLIGONUCLEOTIDE PROBES; ACTIVATED-SLUDGE; DOUGLAS-FIR; OLIGODEOXYNUCLEOTIDE PROBES; PSEUDOMONAS-PUTIDA; DUAL INOCULATION; LACCARIA BICOLOR; MICROBIAL-CELLS; HELPER BACTERIA; RNA
ISSN (print) / ISBN
0940-6360
e-ISSN
1432-1890
Journal
Mycorrhiza
Quellenangaben
Volume: 9,
Issue: 5,
Pages: 271-278
Publisher
Springer
Non-patent literature
Publications
Reviewing status
Peer reviewed