PuSH - Publication Server of Helmholtz Zentrum München: Characterization and application of a strain-specific monoclonal antibody against the rhizosphere bacterium <em>Azospirillum brasilense</em> Wa5.

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Schloter, M. ; Bode, W. ; Hartmann, A.

Characterization and application of a strain-specific monoclonal antibody against the rhizosphere bacterium Azospirillum brasilense Wa5.

Monoclon. Antib. Immunodiagn. Immunother. 16, 183-187 (1997)

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A hybridoma cell line producing a rat monoclonal antibody (MAb Bo-33) directed against lipopolysaccharide of Azospirillum brasilense Wa5 has been established and characterized. Whole bacteria were used as immunogens. The number of antigens per cell was about 1500. The number of antigens per cell of reisolates from the rhizosphere of what was similar to the number of antigens of bacteria cultivated in rich medium. The sensitivity of detection using MAb Bo-33 was about 100 bacteria/ml. Therefore, the MAb was suitable for in situ immunofluorescence detection and a sensitive direct quantification of Azospirillum brasilense Wa5 in rhizosphere extracts.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

ISSN (print) / ISBN 0272-457X

e-ISSN 1557-8097

Journal Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

Quellenangaben Volume: 16, Issue: 2, Pages: 183-187

Publisher Mary Ann Liebert

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Soil Ecology (IBOE)
Research Unit Microbe-Plant Interactions (AMP)