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Production and characterization of strain-specific monoclonal antibodies against outer membrane components of Azospirillum brasilense Sp245.
Monoclon. Antib. Immunodiagn. Immunother. 15, 225-232 (1996)
Several hybridoma cell lines producing murine monoclonal antibodies (MAbs) directed against outer membrane components of the Gram-negative rhizosphere bacterium Azospirillum brasilense Sp245 have been established and characterized, Whole bacterial cells were used as immunogens, Among the clones obtained, 14 hybridoma cell lines were selected for further characterization. Eight MAbs were strain-specific and 6 MAbs showed cross-reactivity with a closely related strain Azospirillum brasilense Sp246, According to the biochemical characterization of the antigenic determinants, MAbs were classified into four groups, The corresponding antigens were lipopolysaccharides (class 1) and an outer membrane protein (class 4), which is common to Azospirillum brasilense Sp245 and Azospirillum brasilense Sp246 as wed as two outer membrane proteins (class 2 and class 3) that are characteristic for Azospirillum brasilense Sp245, The number of antigens per cell varied from 4000 (class 1) to 100 (class 4), In each class high affinity MAbs were identified, which made a sensitive direct quantification of Azospirillum brasilense Sp245 possible.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
WHEAT TRITICUM-AESTIVUM; NITROGEN-FIXATION; INOCULATION; PROTEINS; ROOTS; ASSOCIATION; BACTERIA
ISSN (print) / ISBN
0272-457X
e-ISSN
1557-8097
Quellenangaben
Volume: 15,
Issue: 3,
Pages: 225-232
Publisher
Mary Ann Liebert
Non-patent literature
Publications
Reviewing status
Peer reviewed