PuSH - Publication Server of Helmholtz Zentrum München: Construction of <em>umu-fhuA</em> operon fusions to detect genotoxic potential by an antibody-cell surface reaction.

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Stübner, S. ; Schloter, M. ; Moeck, G.S.* ; Coulton, J.W.* ; Ahne, F. ; Hartmann, A.

Construction of umu-fhuA operon fusions to detect genotoxic potential by an antibody-cell surface reaction.

Environ. Toxicol. 9, 285-291 (1994)

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Open Access Green as soon as Postprint is submitted to ZB.

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For the environmental monitoring of genotoxic effects of chemicals, a new test system was developed using immunochemical detection of a cell surface reporter protein of Escherichia coli. A umu-fhuA operon fusion was constructed on plasmid pST12, which is inducible via the SOS system by the mutagenic agent methyl-methane-sulfonate (MMS). The expression of the outer membrane protein FhuA as a reporter was detectable on the cell surface with a monoclonal anti-FhuA antibody. Enzymelinked immunosorbent assay tests and immunofluorescence microscopy revealed that the operon fusion of PST12 led to specific and strong induction of the reporter protein FhuA after genotoxic treatment with MMS.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

ISSN (print) / ISBN 0884-8181

e-ISSN 1098-2256

Journal Environmental Toxicology

Quellenangaben Volume: 9, Issue: 4, Pages: 285-291

Publisher Wiley

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Soil Ecology (IBOE)
Institute of Radiation Biology (ISB)