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Lyubenova, L. ; Götz, C. ; Golan-Goldhirsh, A.* ; Schröder, P.

Direct effect of CD on glutathione s-transferase and glutathione reductase from Calystegia sepium.

Int. J. Phytoremediat. 9, 465-473 (2007)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Interactions between heavy metals, glutathione, glutathione S-transferase (GST), and glutathione reductase (GR) are being investigated by many working groups, but evaluation of the direct effect of Cd2+ on these enzymes in vitro is lacking. We report here the effect of cadmium (10, 50, 100, 250 mu M CdSO4) on partially purified enzymes from Calystegia sepium. Plants were grown under normal field conditions without metals and the enzymes were extracted by Tris buffer and partially purified by ammonium sulphate fractionation and gel filtration. Glutathione S-transferase activity was measured with different substrates, i. e., 1-chloro-2,4-dinitrobenzene (CDNB), p-nitrobenzylchloride (NBC), and the herbicide Fluorodifen. GST activity was significantly lower in leaf compared to stem, flower, and rhizome and the inhibitory effect of Cd was obtained with NBC and Fluorodifen substrates at 250 mu M. There was no effect of Cd on GR activity up to 250 mu M.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Calystegia sepium; heavy metals; glutathione; glutathione S-transferase; CdSO4; cadmium; HEAVY-METALS; HIGHER-PLANTS; SPRUCE CELLS; CADMIUM; METALLOTHIONEIN; DETOXIFICATION; ARABIDOPSIS; INHIBITION; MIXTURE; FAMILY
ISSN (print) / ISBN 1522-6514
e-ISSN 1549-7879
Journal International Journal of Phytoremediation
Quellenangaben Volume: 9, Issue: 6, Pages: 465-473 Article Number: , Supplement: ,
Publisher Taylor & Francis
Reviewing status Peer reviewed
Institute(s) Research Unit Microbe-Plant Interactions (AMP)