PuSH - Publication Server of Helmholtz Zentrum München

Schröder, P. ; Stampfl, A.

Visualization of glutathione conjugation and inducibility of glutathione S-transferases in onion (Allium cepa L.) epidermal tissue.

Z. Naturforsch. C 54, 1033-1041 (1999)
Open Access Green as soon as Postprint is submitted to ZB.
Glutathione conjugation of 1-chloro-2,4-dinitrobenzene, 1,2-dichloro-4-nitrobenzene, NBD-Cl, monobromobimane and monochlorobimane was found to occur in epidermal tissue of onion bulbs (Allium cepa L.). Conjugation required the presence of glutathione S-transferases (GST). In order to follow glutathione conjugation microscopically, bimanes were utilized. Monochlorobimane was converted to a brightly fluorescing conjugate that was shown to be transported to the nucleus before sequestration in the vacuole occurred. GST activity was stimulated as well as induced by several electrophilic xenobiotics, by cycloheximide and by several glutathione conjugates. γ-Glutamylcysteine conjugates that are formed during enzymatic cleavage of glutathione conjugates in plants, were not active as inducers of enzyme activity. In the light of the stimulating effects of xenobiotic glutathione conjugates on GST activity, it is concluded that glutathione conjugates may act as signal molecules.
Additional Metrics?
[➜Log in]
Edit extra informations Login
Publication type Article: Journal article
Document type Scientific Article
Keywords Glutathione conjugate; Glutathione S-transferase; Monochlorobimane; Onion; Xenobiotic detoxification
ISSN (print) / ISBN 0939-5075
Journal Zeitschrift für Naturforschung - Section C
Quellenangaben Volume: 54, Issue: 12, Pages: 1033-1041 Article Number: , Supplement: ,
Publisher Verl. der Zeitschrift für Naturforschung
Reviewing status Peer reviewed
Institute(s) Institute of Soil Ecology (IBOE)
Institute of Molecular Toxicology and Pharmacology (TOX)
Research Unit Microbe-Plant Interactions (AMP)