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May, R.* ; Schröder, P. ; Sandermann, H.

Ex-situ process for treating PAH-contaminated soil with Phanerochaete chrysosporium.

Environ. Sci. Technol. 31, 2626-2633 (1997)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
Based on the known ability of the white rot fungus Phanerochaete chrysosporium to metabolize PAHs, a fungal reactor system with separate soil extraction and fungal incubation units was constructed. The design of the system allowed samples to be easily removed at strategic positions and to ascertain mineralization. The highly contaminated soil (1-2 mm particle diameter), with a total EPA Method 610 concentration of 41 g of PAHs kg(-1), was spiked with [7,10-C-14]benzo[a]pyrene in order to follow the fate of this tracer by HPLC and high-performance gel permeation chromatography. While mineralization amounted to only 2.5%, it was observed that the fungus reduced the total soil PAH concentration by 45% through polymerization processes. For [7,10-C-14] benzo[a] pyrene, a value of 4.9 mg kg(-1) day(-1) or overall 5.5% was obtained. The polymers remained associated with soil, and no monomeric PAHs were detected in the medium. In parallel experiments without soil, high molecular weight polymers could be found in the medium. Sterile soil and medium controls revealed no polymerization. The results were consistent with literature reports that P. chrysosporium converts PAHs primarily to quinones, which have a strong tendency to polymerize. On the basis of the success of this system, scaling up appears to be justifiable.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords POLYCYCLIC AROMATIC-HYDROCARBONS; WHITE-ROT FUNGI; VERATRYL ALCOHOL; ENVIRONMENTAL-POLLUTANTS; EXTRACELLULAR LIGNINASES; DEGRADATION; BIODEGRADATION; PENTACHLOROPHENOL; BENZO(A)PYRENE; BIOREMEDIATION
ISSN (print) / ISBN 0013-936X
e-ISSN 1520-5851
Journal Environmental Science & Technology
Quellenangaben Volume: 31, Issue: 9, Pages: 2626-2633 Article Number: , Supplement: ,
Publisher ACS
Publishing Place Washington, DC
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Biochemical Plant Pathology (BIOP)
Abteilung Expositionskammern