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Stritzker, J.* ; Kirscher, L.* ; Scadeng, M.* ; Deliolanis, N.C. ; Morscher, S. ; Symvoulidis, P. ; Schaefer, K. ; Zhang, Q.* ; Buckel, L.* ; Hess, M.* ; Donat, U.* ; Bradley, W.G.* ; Ntziachristos, V. ; Szalay, A.A.*

Vaccinia virus-mediated melanin production allows MR and optoacoustic deep tissue imaging and laser-induced thermotherapy of cancer.

Proc. Natl. Acad. Sci. U.S.A. 110, 3316-3320 (2013)
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We reported earlier the delivery of antiangiogenic single chain antibodies by using oncolytic vaccinia virus strains to enhance their therapeutic efficacy. Here, we provide evidence that gene-evoked production of melanin can be used as a therapeutic and diagnostic mediator, as exemplified by insertion of only one or two genes into the genome of an oncolytic vaccinia virus strain. We found that produced melanin is an excellent reporter for optical imaging without addition of substrate. Melanin production also facilitated deep tissue optoacoustic imaging as well as MRI. In addition, melanin was shown to be a suitable target for laser-induced thermotherapy and enhanced oncolytic viral therapy. In conclusion, melanin as a mediator for thermotherapy and reporter for different imaging modalities may soon become a versatile alternative to replace fluorescent proteins also in other biological systems. After ongoing extensive preclinical studies, melanin overproducing oncolytic virus strains might be used in clinical trials in patients with cancer.
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Publication type Article: Journal article
Document type Scientific Article
Keywords translational medicine; personalized medicine; diagnosis; photacoustic; whole-body imaging; Gene ; Expression ; Tumors
Language english
Publication Year 2013
HGF-reported in Year 2013
ISSN (print) / ISBN 0027-8424
e-ISSN 1091-6490
Journal Proceedings of the National Academy of Sciences of the United States of America
Quellenangaben Volume: 110, Issue: 9, Pages: 3316-3320 Article Number: , Supplement: ,
Publisher National Academy of Sciences
Reviewing status Peer reviewed
Institute(s) Institute of Biological and Medical Imaging (IBMI)
POF-Topic(s) 30205 - Bioengineering and Digital Health
Research field(s) Enabling and Novel Technologies
PSP Element(s) G-505500-001
PubMed ID 23401518
DOI 10.1073/pnas.1216916110
WOS ID WOS:000315841900031
Scopus ID 84874450604
Erfassungsdatum 2013-03-13
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