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Open Access Green as soon as Postprint is submitted to ZB.
Sequential multilocus fluorescence in situ hybridization can detect complex patterns of increased gene dosage at the single cell level in tissue sections.
Lab. Invest. 81, 1457-1459 (2001)
Although some impressive applications of multicolor fluorescence in situ hybridization (M-FISH) have been demonstrated on cytogenetic preparations, there are no reports of studies that carry over these advanced multitarget techniques to histological sections of tumor tissues in molecular pathology. Despite recent advances in protocols, M-FISH with a simultaneous multicolor painting tool does not seem to be a feasible approach in histological sections, mainly because of the inherent problem of the third dimension, which leads to complex overlays of both fluorescence signals and nuclei of tumor cells. To overcome these technical limitations, we introduce here an innovative and robust method for the detection of multiple targets by interphase FISH in formalin-fixed and paraffin-embedded tissue, called sequential multilocus fluorescence in situ hybridization (SML-FISH).
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
IN-SITU; AMPLIFICATION; ESOPHAGUS; BREAST
ISSN (print) / ISBN
0023-6837
e-ISSN
1530-0307
Journal
Laboratory Investigation
Quellenangaben
Volume: 81,
Issue: 10,
Pages: 1457-1459
Publisher
Nature Publishing Group
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Pathology (PATH)
Institute of Molecular Radiation Biology (IMS)
Institute of Molecular Radiation Biology (IMS)