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Open Access Green as soon as Postprint is submitted to ZB.
Role of STAT3 in glucocorticoid-induced expression of the human IL-10 gene.
Mol. Immunol. 45, 3230-3237 (2008)
In the present report we have determined the molecular mechanisms, which govern the expression of the human IL-10 gene when induced by the glucocorticoid Methyl-Prednisolone (MP). Treatment of cells with MP at 10-6 M will readily induce IL-10 in CD19+ primary B cells and in a human B cell line. Analysis of the IL-10 promoter showed a robust 18-fold induction and demonstrated that a potential GRE motif was not required, while mutation of the -120 STAT-motif strongly reduced MP-induced trans-activation. A strong induction was also seen with a trimeric STAT-motif and over-expression of dominant-negative STAT3 could block MP induction of IL-10 mRNA. Finally, MP treatment induced binding of STAT3 to the promoter as shown by gelshift, supershift and by chromatin-immunoprecipitation. These data show that glucocorticoid-induced expression of the IL-10 gene is mediated by the transcription factor STAT3.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Human; B cells; Cytokines; Inflammation; Transcription factors
ISSN (print) / ISBN
0161-5890
e-ISSN
1872-9142
Journal
Molecular Immunology
Quellenangaben
Volume: 45,
Issue: 11,
Pages: 3230-3237
Publisher
Elsevier
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
CCG Inflammatory Lung Diseases (CPC-KEL)
Research Unit Gene Vector (AGV)
Research Unit Gene Vector (AGV)