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Open Access Gold as soon as Publ. Version/Full Text is submitted to ZB.
Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells.
EMBO Rep. 8, 1052-1060 (2007)
Members of the Argonaute (Ago) protein family associate with small RNAs and have important roles in RNA silencing. Here, we analysed Ago1- and Ago2-containing protein complexes in human cells. Separation of Ago-associated messenger ribonucleoproteins (mRNPs) showed that Ago1 and Ago2 reside in three complexes with distinct Dicer and RNA-induced silencing complex activities. A comprehensive proteomic analysis of Ago-containing mRNPs identified a large number of proteins involved in RNA metabolism. By using co-immunoprecipitation experiments followed by RNase treatment, we biochemically mapped interactions within Ago mRNPs. Using reporter assays and knockdown experiments, we showed that the putative RNA-binding protein RBM4 is required for microRNA-guided gene regulation.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Argonaute proteins; gene silencing; microRNA; RNA interference
ISSN (print) / ISBN
1469-221X
e-ISSN
1469-3178
Journal
EMBO Reports
Quellenangaben
Volume: 8,
Issue: 11,
Pages: 1052-1060
Publisher
EMBO Press
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Molecular Immunology (IMI)