OpenSSL SSL_connect: Connection reset by peer in connection to v2.sherpa.ac.uk:443 PuSH - Publication Server of Helmholtz Zentrum München: Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells.

PuSH - Publication Server of Helmholtz Zentrum München

Höck, J.* ; Weinmann, L.* ; Ender, C.* ; Rüdel, S.* ; Kremmer, E. ; Raabe, M.* ; Urlaub, H.* ; Meister, G.*

Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells.

EMBO Rep. 8, 1052-1060 (2007)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Members of the Argonaute (Ago) protein family associate with small RNAs and have important roles in RNA silencing. Here, we analysed Ago1- and Ago2-containing protein complexes in human cells. Separation of Ago-associated messenger ribonucleoproteins (mRNPs) showed that Ago1 and Ago2 reside in three complexes with distinct Dicer and RNA-induced silencing complex activities. A comprehensive proteomic analysis of Ago-containing mRNPs identified a large number of proteins involved in RNA metabolism. By using co-immunoprecipitation experiments followed by RNase treatment, we biochemically mapped interactions within Ago mRNPs. Using reporter assays and knockdown experiments, we showed that the putative RNA-binding protein RBM4 is required for microRNA-guided gene regulation.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Argonaute proteins; gene silencing; microRNA; RNA interference
ISSN (print) / ISBN 1469-221X
e-ISSN 1469-3178
Journal EMBO Reports
Quellenangaben Volume: 8, Issue: 11, Pages: 1052-1060 Article Number: , Supplement: ,
Publisher EMBO Press
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Molecular Immunology (IMI)