Home
Deutsch
Advanced Search
Browse by ...
Publication overview
Contact persons
Help
DOI
PMC
 |
|
Open Access Green as soon as Postprint is submitted to ZB.
Liposome-mediated gene transfer to fetal human ventral mesencephalic explant cultures.
Neurosci. Lett. 308, 169-172 (2001)
The feasibility of non-viral gene transfer using liposomes is described for human fetal nigral tissue. Ventral mesencephalic explants from 6 to 12 week old fetuses were grown as free-floating roller tube cultures. For the transfection, a vector coding for beta -galactosidase driven by the Rous Sarcoma Virus promoter was used. The developmental stage of the human tissue, time in vitro and the amount of vector DNA used significantly influenced the transfection efficiency. Optimal transfection results were obtained with tissue from a 10 week old fetus, cultured for 4 days and transfected with mixtures containing 4 mug vector DNA. Histological analysis suggested that a specific population of ventral mesencephalic precursor cells were the target for the gene transfer. This finding might have implications for gene delivery and cell replacement strategies in Parkinson's disease.
Altmetric
Additional Metrics?
Edit extra informations
Login
Publication type
Article: Journal article
Document type
Scientific Article
Keywords
human; development; ventral mesencephalon; tissue culture; gene transfer; liposomes; progenitor cells; dopamine; CENTRAL-NERVOUS-SYSTEM; STEM-CELLS; DOPAMINERGIC-NEURONS; RAT-BRAIN; EXPRESSION; SURVIVAL; MODEL; DNA
ISSN (print) / ISBN
0304-3940
e-ISSN
0304-3940
Journal
Neuroscience Letters
Quellenangaben
Volume: 308,
Issue: 3,
Pages: 169-172
Publisher
Elsevier
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Human Genetics (IHG)