OpenSSL SSL_connect: Connection reset by peer in connection to v2.sherpa.ac.uk:443 PuSH - Publication Server of Helmholtz Zentrum München: Development of a high density 600K SNP genotyping array for chicken.

PuSH - Publication Server of Helmholtz Zentrum München

Kranis, A.* ; Gheyas, A.A.* ; Boschiero, C.* ; Turner, F.* ; Yu, L.* ; Smith, S.* ; Talbot, R.* ; Pirani, A.* ; Brew, F.* ; Kaiser, P.* ; Hocking, P.M.* ; Fife, M.* ; Salmon, N.* ; Fulton, J.* ; Strom, T.M. ; Haberer, G. ; Weigend, S.* ; Preisinger, R.* ; Gholami, M.* ; Qanbari, S.* ; Simianer, H.* ; Watson, K.A.* ; Woolliams, J.A.* ; Burt, D.W.*

Development of a high density 600K SNP genotyping array for chicken.

BMC Genomics 14:59 (2013)
Publ. Version/Full Text Volltext DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Background: High density (HD) SNP genotyping arrays are an important tool for genetic analyses of animals and plants. Although the chicken is one of the most important farm animals, no HD array is yet available for high resolution genetic analysis of this species. Results: We report here the development of a 600 K Affymetrix(R) Axiom(R) HD genotyping array designed using SNPs segregating in a wide variety of chicken populations. In order to generate a large catalogue of segregating SNPs, we re-sequenced 243 chickens from 24 chicken lines derived from diverse sources (experimental, commercial broiler and layer lines) by pooling 10-15 samples within each line. About 139 million (M) putative SNPs were detected by mapping sequence reads to the new reference genome (Gallus_gallus_4.0) of which similar to 78 M appeared to be segregating in different lines. Using criteria such as high SNP-quality score, acceptable design scores predicting high conversion performance in the final array and uniformity of distribution across the genome, we selected similar to 1.8 M SNPs for validation through genotyping on an independent set of samples (n = 282). About 64% of the SNPs were polymorphic with high call rates (>98%), good cluster separation and stable Mendelian inheritance. Polymorphic SNPs were further analysed for their population characteristics and genomic effects. SNPs with extreme breach of Hardy-Weinberg equilibrium (P < 0.00001) were excluded from the panel. The final array, designed on the basis of these analyses, consists of 580,954 SNPs and includes 21,534 coding variants. SNPs were selected to achieve an essentially uniform distribution based on genetic map distance for both broiler and layer lines. Due to a lower extent of LD in broilers compared to layers, as reported in previous studies, the ratio of broiler and layer SNPs in the array was kept as 3:2. The final panel was shown to genotype a wide range of samples including broilers and layers with over 100 K to 450 K informative SNPs per line. A principal component analysis was used to demonstrate the ability of the array to detect the expected population structure which is an important pre-investigation step for many genome-wide analyses. Conclusions: This Affymetrix(R) Axiom(R) array is the first SNP genotyping array for chicken that has been made commercially available to the public as a product. This array is expected to find widespread usage both in research and commercial application such as in genomic selection, genome-wide association studies, selection signature analyses, fine mapping of QTLs and detection of copy number variants.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Genotyping Array ; Chicken ; Snp; Linkage Disequilibrium ; Genetic Diversity ; Breeds ; Selection ; Sequence ; Provide ; Markers ; Extent ; Map
ISSN (print) / ISBN 1471-2164
e-ISSN 1471-2164
Journal BMC Genomics
Quellenangaben Volume: 14, Issue: 1, Pages: , Article Number: 59 Supplement: ,
Publisher BioMed Central
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Human Genetics (IHG)
Institute of Bioinformatics and Systems Biology (IBIS)