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Open Access Green as soon as Postprint is submitted to ZB.
Analysis of chromosomal alterations in non-small cell lung cancer by multiplex-FISH, comparative genomic hybridization, and multicolor bar coding.
Lab. Invest. 80, 1031-1041 (2000)
Lung cancer has a considerable impact on morbidity and mortality throughout the world. Despite extensive effort, no lung cancer-specific cytogenetic changes, such as lineage-specific translocations or inversions, have been described to date. In this study we used multiplex fluorescence in situ hybridization (M-FISH), comparative genomic hybridization, and multicolor bar coding to analyze eight cell lines derived from non-small cell lung cancers. M-FISH did not identify any balanced translocations, which are the dominating feature in leukemias and lymphomas. Instead, M-FISH unraveled an enormous number of numerical and structural aberrations, with each tumor having its own "private" pattern of chromosomal changes. In contrast, comparative genomic hybridization demonstrated similarities between tumors, because each cell line shared some chromosomal segments that were commonly gained or lost. One of these involved chromosome 12. Chromosome 12 specific bar code probe sets were constructed and used to demonstrate that breaks on chromosome 12 occur preferentially within specific bands. With the progressive use of higher resolution approaches, more information can be gained about the chromosomal alterations in cancer.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
0023-6837
e-ISSN
1530-0307
Journal
Laboratory Investigation
Quellenangaben
Volume: 80,
Issue: 7,
Pages: 1031-1041
Publisher
Nature Publishing Group
Reviewing status
Peer reviewed
Institute(s)
Institute of Human Genetics (IHG)