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Open Access Green as soon as Postprint is submitted to ZB.
Activated B cells mediate efficient expansion of rare antigen-specific T cells.
Hum. Immunol. 68, 75-85 (2007)
Potent professional antigen-presenting cells (APC) are essential tools to activate and expand antigen-specific T cells in vitro for use in adoptive immunotherapy. CD40-activated B cells can be easily generated and propagated from human donors and have been successfully used to generate antigen-specific T-cell cultures. Here we show that CD40-activated B cells strongly and specifically expand rare populations of antigen-specific CD8 T cells, with frequencies of less than 1 in 20,000 CD8 T cells in peripheral blood. We focused on T cells recognizing an epitope from the human papillomavirus 16 (HPV-16) E7 protein. In 6 of 6 healthy donors, epitope-specific CD8+ T cells were found to be "rare" by this criterion, as shown by staining with human leukocyte antigen (HLA)/peptide multimers. Using peptide-loaded CD40-activated B cells, epitope-specific T cells could be selectively expanded in all donors up to 10(6) fold, and the resulting T-cell cultures contained up to 88% specific T cells. These results strongly encourage the use of CD40-stimulated B cells as APCs in immunotherapy.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Activated B cells; antigen-presenting cells; CD8 T cells; immunotherapy; HLA/peptide tetramers
ISSN (print) / ISBN
0198-8859
e-ISSN
0198-8859
Journal
Human Immunology
Quellenangaben
Volume: 68,
Issue: 2,
Pages: 75-85
Publisher
Elsevier
Non-patent literature
Publications
Reviewing status
Peer reviewed