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Zentz, C. ; Wiesner, M. ; Man, S.* ; Frankenberger, B. ; Wollenberg, B.* ; Hillemanns, P.* ; Zeidler, R.* ; Hammerschmidt, W. ; Moosmann, A.

Activated B cells mediate efficient expansion of rare antigen-specific T cells.

Hum. Immunol. 68, 75-85 (2007)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Potent professional antigen-presenting cells (APC) are essential tools to activate and expand antigen-specific T cells in vitro for use in adoptive immunotherapy. CD40-activated B cells can be easily generated and propagated from human donors and have been successfully used to generate antigen-specific T-cell cultures. Here we show that CD40-activated B cells strongly and specifically expand rare populations of antigen-specific CD8 T cells, with frequencies of less than 1 in 20,000 CD8 T cells in peripheral blood. We focused on T cells recognizing an epitope from the human papillomavirus 16 (HPV-16) E7 protein. In 6 of 6 healthy donors, epitope-specific CD8+ T cells were found to be "rare" by this criterion, as shown by staining with human leukocyte antigen (HLA)/peptide multimers. Using peptide-loaded CD40-activated B cells, epitope-specific T cells could be selectively expanded in all donors up to 10(6) fold, and the resulting T-cell cultures contained up to 88% specific T cells. These results strongly encourage the use of CD40-stimulated B cells as APCs in immunotherapy.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Activated B cells; antigen-presenting cells; CD8 T cells; immunotherapy; HLA/peptide tetramers
Language english
Publication Year 2007
HGF-reported in Year 2007
ISSN (print) / ISBN 0198-8859
e-ISSN 0198-8859
Journal Human Immunology
Quellenangaben Volume: 68, Issue: 2, Pages: 75-85 Article Number: , Supplement: ,
Publisher Elsevier
Reviewing status Peer reviewed
Institute(s) Research Unit Gene Vector (AGV)
Institute of Molecular Immunology (IMI)
POF-Topic(s) 30203 - Molecular Targets and Therapies
30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
Research field(s) Immune Response and Infection
PSP Element(s) G-501500-001
G-501700-001
PubMed ID 17321896
DOI 10.1016/j.humimm.2006.12.004
WOS ID 000244799300001
Scopus ID 33847100649
Erfassungsdatum 2007-11-15
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