PuSH - Publication Server of Helmholtz Zentrum München: Evaluating the assignment of <em>alk</em>B terminal restriction fragments and sequence types to distinct bacterial taxa.

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Giebler, J.* ; Wick, L.Y.* ; Schloter, M. ; Harms, H.* ; Chatzinotas, A.*

Evaluating the assignment of alkB terminal restriction fragments and sequence types to distinct bacterial taxa.

Appl. Environ. Microbiol. 79, 3129-3132 (2013)

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Open Access Green as soon as Postprint is submitted to ZB.

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Sequence and terminal restriction fragment length polymorphism (T-RFLP) analyses revealed multiple alkB gene copies/cell in soil bacterial isolates and an apparently high genetic mobility among various phylogenetic groups. Identifying alkane degraders by alkB terminal restriction fragments (T-RFs) and sequences is strongly biased, as the phylogenetic trees based on 16S rRNA and alkB gene sequences were highly inconsistent.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Length-polymorphism Analysis ; Alkane Hydroxylase Genes ; Community Structure ; Pseudomonas ; Degradation ; Pcr ; Plasmid

ISSN (print) / ISBN 0099-2240

e-ISSN 1098-5336

Journal Applied and Environmental Microbiology

Quellenangaben Volume: 79, Issue: 9, Pages: 3129-3132

Publisher American Society for Microbiology (ASM)

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Research Unit Comparative Microbiome Analysis (COMI)