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Weinert, N.* ; Meincke, R.* ; Gottwald, C.* ; Heuer, H.* ; Gomes, N.C.M.* ; Schloter, M. ; Berg, G.* ; Smalla, K.

Rhizosphere communities of genetically modified zeaxanthin-accumulating potato plants and their parent cultivar differ less than those of different potato cultivars.

Appl. Environ. Microbiol. 75, 3859-3865 (2009)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
The effects of genetically modified (GM), zeaxanthin-accumulating potato plants on microbial communities in the rhizosphere were compared to the effects of different potato cultivars. Two GM lines and their parental cultivar, as well as four other potato cultivars, were grown in randomized field plots at two sites and in different years. Rhizosphere samples were taken at three developmental stages during plant growth and analyzed using denaturing gradient gel electrophoresis (DGGE) fingerprints of Bacteria, Actinobacteria, Alpha- and Betaproteobacteria, Bacillus, Streptomycetaceae, Pseudomonas, gacA, Fungi, and Ascomycetes. In the bacterial DGGE gels analyzed, significant differences between the parental cultivar and the two GM lines were detected mainly for Actinobacteria but also for Betaproteobacteria and Streptomycetaceae, yet these differences occurred only at one site and in one year. Significant differences occurred more frequently for Fungi, especially Ascomycetes, than for bacteria. When all seven plant genotypes were compared, DGGE analysis revealed that different cultivars had a greater effect on both bacterial and fungal communities than genetic modification. The effects of genetic modification were detected mostly at the senescence developmental stage of the plants. The site was the overriding factor affecting microbial community structure compared to the plant genotype. In general, the fingerprints of the two GM lines were more similar to that of the parental cultivar, and the differences observed did not exceed natural cultivar-dependent variability.
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Publication type Article: Journal article
Document type Scientific Article
Keywords gradient gel-electrophoresis; soil bacterial community; maize zea-mays; microbial communities; macular degeneration; diversity; microorganisms; similarity; roots
ISSN (print) / ISBN 0099-2240
e-ISSN 1098-5336
Journal Applied and Environmental Microbiology
Quellenangaben Volume: 75, Issue: 12, Pages: 3859-3865 Article Number: , Supplement: ,
Publisher American Society for Microbiology (ASM)
Reviewing status Peer reviewed
Institute(s) Institute of Soil Ecology (IBOE)