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Lamacchia, M.* ; Clayton, J.R.* ; Wang-Sattler, R. ; Steinmetz, L.M.* ; Levashina, E.A.* ; Blandin, S.A.*

Silencing of genes and alleles by RNAi in Anopheles gambiae.

Methods Mol. Biol. 923, 161-176 (2013)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Anopheles gambiae mosquitoes are the major vectors of human malaria parasites. However, mosquitoes are not passive hosts for parasites, actively limiting their development in vivo. Our current understanding of the mosquito antiparasitic response is mostly based on the phenotypic analysis of gene knockdowns obtained by RNA interference (RNAi), through the injection or transfection of long dsRNAs in adult mosquitoes or cultured cells, respectively. Recently, RNAi has been extended to silence specifically one allele of a given gene in a heterozygous context, thus allowing to compare the contribution of different alleles to a phenotype in the same genetic background.
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Publication type Article: Journal article
Document type Scientific Article
Editors Menard, R.*
Corresponding Author
Keywords Malaria; Mosquito; RNAi; Reciprocal allele-specific RNAi; Real-time PCR; Western blot
ISSN (print) / ISBN 1064-3745
e-ISSN 1940-6029
Conference Title Malaria : Methods and Protocols
Journal Methods in Molecular Biology
Quellenangaben Volume: 923, Issue: , Pages: 161-176 Article Number: , Supplement: ,
Publisher Springer
Publishing Place Berlin [u.a.]
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Research Unit Molecular Epidemiology (AME)