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Open Access Green as soon as Postprint is submitted to ZB.
Silencing of genes and alleles by RNAi in Anopheles gambiae.
Methods Mol. Biol. 923, 161-176 (2013)
Anopheles gambiae mosquitoes are the major vectors of human malaria parasites. However, mosquitoes are not passive hosts for parasites, actively limiting their development in vivo. Our current understanding of the mosquito antiparasitic response is mostly based on the phenotypic analysis of gene knockdowns obtained by RNA interference (RNAi), through the injection or transfection of long dsRNAs in adult mosquitoes or cultured cells, respectively. Recently, RNAi has been extended to silence specifically one allele of a given gene in a heterozygous context, thus allowing to compare the contribution of different alleles to a phenotype in the same genetic background.
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Publication type
Article: Journal article
Document type
Scientific Article
Editors
Menard, R.*
Keywords
Malaria; Mosquito; RNAi; Reciprocal allele-specific RNAi; Real-time PCR; Western blot
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Conference Title
Malaria : Methods and Protocols
Journal
Methods in Molecular Biology
Quellenangaben
Volume: 923,
Pages: 161-176
Publisher
Springer
Publishing Place
Berlin [u.a.]
Reviewing status
Peer reviewed
Institute(s)
Research Unit Molecular Epidemiology (AME)