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Open Access Green as soon as Postprint is submitted to ZB.
The pattern-recognition molecule Nod1 is localized at the plasma membrane at sites of bacterial interaction.
Cell. Microbiol. 10, 477-486 (2008)
The pattern-recognition molecule Nod1 is a critical sensor for bacterial derived diaminopimelic acid-containing peptidoglycan fragments which induces innate immune responses in epithelial cells. Here we report the subcellular localization of this protein in human epithelial cells. Nod1 is localized in the cytosol and at the plasma membrane in human cells. This membrane association is dependent on the integrity of the protein, on its signalling capacity and on an intact actin cytoskeleton. Signalling-inactive mutants of Nod1 or disruption of the actin cytoskeleton interferes with this localization pattern and impacts on downstream NF-kappaB activation. Moreover, the invasive bacterium Shigella flexneri was used as a model for physiological activation of Nod1. Imaging revealed that Nod1 is recruited to the site of bacterial entry, where it colocalizes with NEMO. Our data provide evidence that membrane association is linked to Nod1 function and, in view of recent findings on Nod2, that this may be a common feature of NLR family members.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
1462-5814
e-ISSN
1462-5822
Journal
Cellular Microbiology
Quellenangaben
Volume: 10,
Issue: 2,
Pages: 477-486
Publisher
Wiley
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Molecular Immunology (IMI)