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Behnke, K. ; Ghirardo, A. ; Janz, D.* ; Kanawati, B. ; Esperschütz, J. ; Zimmer, I. ; Schmitt-Kopplin, P. ; Niinemets, U.* ; Polle, A.* ; Schnitzler, J.-P. ; Rosenkranz, M.

Isoprene function in two contrasting poplars under salt and sunflecks.

Tree Physiol. 33, 562-578 (2013)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
In the present study, biogenic volatile organic compound (BVOC) emissions and photosynthetic gas exchange of salt-sensitive (Populus x canescens (Aiton) Sm.) and salt-tolerant (Populus euphratica Oliv.) isoprene-emitting and non-isoprene-emitting poplars were examined under controlled high-salinity and high-temperature and -light episode ('sunfleck') treatments. Combined treatment with salt and sunflecks led to an increased isoprene emission capacity in both poplar species, although the photosynthetic performance of P. × canescens was reduced. Indeed, different allocations of isoprene precursors between the cytosol and the chloroplast in the two species were uncovered by means of (13)CO2 labeling. Populus × canescens leaves, moreover, increased their use of 'alternative' carbon (C) sources in comparison with recently fixed C for isoprene biosynthesis under salinity. Our studies show, however, that isoprene itself does not have a function in poplar survival under salt stress: the non-isoprene-emitting leaves showed only a slightly decreased photosynthetic performance compared with wild type under salt treatment. Lipid composition analysis revealed differences in the double bond index between the isoprene-emitting and non-isoprene-emitting poplars. Four clear metabolomics patterns were recognized, reflecting systemic changes in flavonoids, sterols and C fixation metabolites due to the lack/presence of isoprene and the absence/presence of salt stress. The studies were complemented by long-term temperature stress experiments, which revealed the thermotolerance role of isoprene as the non-isoprene-emitting leaves collapsed under high temperature, releasing a burst of BVOCs. Engineered plants with a low isoprene emission potential might therefore not be capable of resisting high-temperature episodes.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords 13C labeling; Populus euphratica; Populus × canescens; isoprene; lipids; metabolomics; salt; thermotolerance; Organic-compound Emissions ; Oak Quercus-robur ; Water-stress ; Increases Thermotolerance ; Volatile Isoprenoids ; Electrolyte Leakage ; Synthase Activity ; Energy Crops ; Fatty-acids ; Leaves
ISSN (print) / ISBN 0829-318X
e-ISSN 1758-4469
Journal Tree Physiology
Quellenangaben Volume: 33, Issue: 6, Pages: 562-578 Article Number: , Supplement: ,
Publisher Oxford University Press
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Research Unit Environmental Simulation (BIOP-EUS)
Research Unit Analytical BioGeoChemistry (BGC)
Institute of Soil Ecology (IBOE)