Home
Deutsch
Advanced Search
Browse by ...
Publication overview
Contact persons
Help
DOI
PMC
 |
|
Open Access Green as soon as Postprint is submitted to ZB.
Foxa2-venus fusion reporter mouse line allows live-cell analysis of endoderm-derived organ formation.
Genesis 51, 596-604 (2013)
The Foxa2-winged helix/forkhead box transcription factor (TF) is absolutely required for endoderm formation and organogenesis. Foxa2 plays essential roles during lung, liver, pancreas, and gastrointestinal tract development and regulates cell-type specific programs in the adult organism. To specifically address Foxa2 function during organ development and homeostasis, we generated a Foxa2-Venus fusion (FVF) reporter protein by gene targeting in embryonic stem (ES) cells. The FVF knock-in reporter is expressed under endogenous Foxa2 control and the fluorescent protein fusion does not interfere with TF function, as homozygous mice are viable and fertile. Moreover, the FVF protein localizes to the nucleus, associates with chromatin during mitosis, and reflects the endogenous Foxa2 protein distribution pattern in several tissues in heterozygous animals. Importantly, live-cell imaging on single-cell level of the FVF and Sox17-Cherry fusion double knock-in reporter ES cell line reveals the dynamics of endoderm TF accumulation during ES cell differentiation. The FVF reporter also allowed us to identify the endoderm progenitors during gastrulation and to visualize the different branching morphogenesis modes of the lung and pancreas epithelium in ex vivo embryo and organ cultures. In summary, the generation of the FVF reporter line adds an important new tool to visualize and analyse endoderm-derived organ development and homeostasis on the cellular and molecular level.
Altmetric
Additional Metrics?
Edit extra informations
Login
Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Foxa2; Sox17; ES cells; Mouse embryo; Gastrulation; Fluorescent reporter; Endoderm; Node; Notochord; Liver; Lung; Pancreas; Gastrointestinal tract; Transcription Factor ; Definitive Endoderm ; Cre Recombinase ; Fork-head ; Embryo ; Expression ; Notochord ; Differentiation ; Hnf-3-beta ; Genes
ISSN (print) / ISBN
1526-954X
e-ISSN
1526-968X
Quellenangaben
Volume: 51,
Issue: 8,
Pages: 596-604
Publisher
Wiley
Non-patent literature
Publications
Reviewing status
Peer reviewed