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Kronsbein, H.C.* ; Jastorff, A.M.* ; Maccarrone, G.* ; Stalla, G.* ; Wurst, W. ; Holsboer, F.* ; Turck, C.W.* ; Deussing, J.M.*

CRHR1-dependent effects on protein expression and posttranslational modification in AtT-20 cells.

Mol. Cell. Endocrinol. 292, 1-10 (2008)

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Open Access Green as soon as Postprint is submitted to ZB.

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Corticotropin-releasing hormone (CRH) plays a major role in coordinating the organism's stress response, including the activity of the hypothalamic-pituitary-adrenocortical axis. The molecular underpinnings of CRH-dependent signal transduction mechanisms in the anterior pituitary have not yet been revealed in detail. In order to dissect the signal transduction cascades activated by CRH receptor type 1, a comparative proteome approach was performed in vitro utilizing murine corticotroph AtT-20 cells. Alterations in protein expression and posttranslational modification in response to CRH stimulation were studied by 2D gel electrophoresis. Selected candidates were analyzed by immunoblotting and quantitative real-time PCR. The differential analyses revealed proteins regulated or modified related to diverse cellular processes. Amongst others we identified alterations in PRKAR1A, the regulatory subunit of protein kinase A; in PGK1 and PGAM1, key regulators of glycolysis; and in proteins involved in proteasome-mediated proteolysis, PSMC2 and PSMA3. These results offer novel entry points to molecular mechanisms underlying stress responses elicited via the hypothalamic-pituitary-adrenocortical axis.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords hypothalamic-pituitary-adrenocortical axis; corticotropin-releasing hormone; corticotropin-releasing hormone receptor; type 1; AtT-20 cells; proteome analysis

ISSN (print) / ISBN 0303-7207

e-ISSN 1872-8057

Journal Molecular and Cellular Endocrinology

Quellenangaben Volume: 292, Issue: 1-2, Pages: 1-10

Publisher Elsevier

Publishing Place Shannon

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Developmental Genetics (IDG)