OpenSSL SSL_connect: Connection reset by peer in connection to v2.sherpa.ac.uk:443 PuSH - Publication Server of Helmholtz Zentrum München: <em>In vitro</em> monitoring of base excision repair in <em>Saccharomyces cerevisiae</em>.

PuSH - Publication Server of Helmholtz Zentrum München

Mörtl, S. ; Ahne, F.

In vitro monitoring of base excision repair in Saccharomyces cerevisiae.

Methods Mol. Biol. 920, 279-287 (2012)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Base excision repair (BER) is an important mechanism to maintain genomic stability. Here we offer a set of protocols to quantitatively analyze BER capacity in whole cell-free yeast extracts. Cell-free yeast extracts were obtained by a French press procedure and repair capacities were measured by using oligonucleotide substrates. Repair products were separated by polyacrylamide gel electrophoresis and detected by autoradiography. These set of methods allow the analysis of different kinds of base damage and of individual mechanistic steps within BER. We used these protocols to investigate a new role of the DNA double strand break repair protein XRS1 in BER (1).
Altmetric
Additional Metrics?
[➜Log in]
Edit extra informations Login
Publication type Article: Journal article
Document type Scientific Article
Editors Bjergbaek, L.*
Corresponding Author
Keywords Base excision repair assay; Incision assay; Gap filling assay; Whole cell-free extracts; Radioactive labeling; Oligonucleotide-based assays; Denaturing polyacrylamide gel electrophoresis
ISSN (print) / ISBN 1064-3745
e-ISSN 1940-6029
Conference Title DNA Repair Protocols
Journal Methods in Molecular Biology
Quellenangaben Volume: 920, Issue: , Pages: 279-287 Article Number: , Supplement: ,
Publisher Springer
Publishing Place Berlin [u.a.]
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Radiation Biology (ISB)