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Open Access Green as soon as Postprint is submitted to ZB.
In vitro monitoring of base excision repair in Saccharomyces cerevisiae.
Methods Mol. Biol. 920, 279-287 (2012)
Base excision repair (BER) is an important mechanism to maintain genomic stability. Here we offer a set of protocols to quantitatively analyze BER capacity in whole cell-free yeast extracts. Cell-free yeast extracts were obtained by a French press procedure and repair capacities were measured by using oligonucleotide substrates. Repair products were separated by polyacrylamide gel electrophoresis and detected by autoradiography. These set of methods allow the analysis of different kinds of base damage and of individual mechanistic steps within BER. We used these protocols to investigate a new role of the DNA double strand break repair protein XRS1 in BER (1).
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Publication type
Article: Journal article
Document type
Scientific Article
Editors
Bjergbaek, L.*
Keywords
Base excision repair assay; Incision assay; Gap filling assay; Whole cell-free extracts; Radioactive labeling; Oligonucleotide-based assays; Denaturing polyacrylamide gel electrophoresis
Language
english
Publication Year
2012
HGF-reported in Year
0
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Conference Title
DNA Repair Protocols
Journal
Methods in Molecular Biology
Quellenangaben
Volume: 920,
Pages: 279-287
Publisher
Springer
Publishing Place
Berlin [u.a.]
Reviewing status
Peer reviewed
Institute(s)
Institute of Radiation Biology (ISB)
POF-Topic(s)
30202 - Environmental Health
Research field(s)
Radiation Sciences
PSP Element(s)
G-500200-001
PubMed ID
22941610
Erfassungsdatum
2013-08-05