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Hartl, D.* ; Irmler, M. ; Römer, I.* ; Mader, M.T. ; Mao, L.* ; Zabel, C.* ; Hrabě de Angelis, M. ; Beckers, J. ; Klose, J.*

Transcriptome and proteome analysis of early embryonic mouse brain development.

Proteomics 8, 1257-1265 (2008)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Mouse embryonic brain development involves sequential differentiation of multipotent progenitors into neurons and glia cells. Using microarrays and large 2-DE, we investigated the mouse brain transcriptome and proteome of embryonic days 9.5, 11.5, and 13.5. During this developmental period, neural progenitor cells shift from proliferation to neuronal differentiation. As expected, we detected numerous expression changes between all time points investigated, but interestingly, the rate of alteration remained in a similar range within 2 days of development. Furthermore, up- and down-regulation of gene products was balanced at each time point which was also seen at embryonic days 16-18. We hypothesize that during embryonic development, the rate of gene expression alteration is rather constant due to limited cellular resources such as energy, space, and free water. A similar complexity in terms of expressed genes and proteins suggests that changes in relative concentrations rather than an increase in the number of gene products dominate cellular differentiation. In general, expression of metabolism and cell cycle related gene products was down-regulated when precursor cells switched from proliferation to neuronal differentiation (days 9.5-11.5), whereas neuron specific gene products were up-regulated. A detailed functional analysis revealed their implication in differentiation related processes such as rearrangement of the actin cytoskeleton as well as Notch- and Wnt-signaling pathways.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Differentiation; Embryonic brain development; Neural progenitor cells; Transcriptome
ISSN (print) / ISBN 1615-9853
e-ISSN 1615-9861
Journal Proteomics
Quellenangaben Volume: 8, Issue: 6, Pages: 1257-1265 Article Number: , Supplement: ,
Publisher Wiley
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Experimental Genetics (IEG)
Institute of Stem Cell Research (ISF)