PuSH - Publication Server of Helmholtz Zentrum München: The PI3K/Akt/mTOR pathway is implicated in the premature senescence of primary human endothelial cells exposed to chronic radiation.

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Yentrapalli, R. ; Azimzadeh, O. ; Sriharshan, A. ; Malinowsky, K.* ; Merl, J. ; Wojcik, A.* ; Harms-Ringdahl, M.* ; Atkinson, M.J. ; Becker, K.-F.* ; Haghdoost, S.* ; Tapio, S.

The PI3K/Akt/mTOR pathway is implicated in the premature senescence of primary human endothelial cells exposed to chronic radiation.

PLoS ONE 8:e70024 (2013)

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	Open Access Gold

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The etiology of radiation-induced cardiovascular disease (CVD) after chronic exposure to low doses of ionizing radiation is only marginally understood. We have previously shown that a chronic low-dose rate exposure (4.1 mGy/h) causes human umbilical vein endothelial cells (HUVECs) to prematurely senesce. We now show that a dose rate of 2.4 mGy/h is also able to trigger premature senescence in HUVECs, primarily indicated by a loss of growth potential and the appearance of the senescence-associated markers ß-galactosidase (SA-ß-gal) and p21. In contrast, a lower dose rate of 1.4 mGy/h was not sufficient to inhibit cellular growth or increase SA-ß-gal-staining despite an increased expression of p21. We used reverse phase protein arrays and triplex Isotope Coded Protein Labeling with LC-ESI-MS/MS to study the proteomic changes associated with chronic radiation-induced senescence. Both technologies identified inactivation of the PI3K/Akt/mTOR pathway accompanying premature senescence. In addition, expression of proteins involved in cytoskeletal structure and EIF2 signaling was reduced. Age-related diseases such as CVD have been previously associated with increased endothelial cell senescence. We postulate that a similar endothelial aging may contribute to the increased rate of CVD seen in populations chronically exposed to low-dose-rate radiation.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Pleckstrin Homology Domain ; Protein-kinase B ; Progenitor Cells ; Ionizing-radiation ; Replicative Senescence ; Proteomic Analysis ; Telomerase Inactivation ; Accelerates Senescence ; Mortality Experience ; Label-free

ISSN (print) / ISBN 1932-6203

Journal PLoS ONE

Quellenangaben Volume: 8, Issue: 8, Article Number: e70024

Publisher Public Library of Science (PLoS)

Publishing Place Lawrence, Kan.

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Radiation Biology (ISB)
CF Metabolomics & Proteomics (CF-MPC)