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Open Access Green as soon as Postprint is submitted to ZB.
Peptidomic analysis of rat urine using capillary electrophoresis coupled to mass spectrometry.
Proteomics Clin. Appl. 1, 650-660 (2007)
We have established and validated a protocol for the peptidomic analysis of rat urine using CE coupled to MS (CE-MS). In the first experiments, the reproducibility of the CE-MS set-up and of the established preparation procedure were assessed. To establish a first rat urinary peptidome map, samples were also analyzed using CE-FT-ICR. The subsequent analysis of independent samples from two different strains (WISTAR and CD) indicated strain-specific differences, which were validated in a blinded assessment. MS/MS revealed the presence of specific fragments from well-known urinary rat peptides, such as collagens, alpha-1-antitrypsin, and serum albumin. The CE-MS-based peptidomics platform may provide novel insights into body fluids of animal models, such as rat or mice. Together with peptide identification, the technology appears to be an excellent, complimentary, and non-invasive tool to analyze toxicological or other (patho)physiological effects of pharmaceutical compounds in animal models.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Biomarker; CE-MS; Rat; Urine
ISSN (print) / ISBN
1862-8346
e-ISSN
1862-8354
Quellenangaben
Volume: 1,
Issue: 7,
Pages: 650-660
Publisher
Wiley
Publishing Place
Weinheim
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Ecological Chemistry (IOEC)