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Coon, J.J.* ; Zürbig, P.* ; Dakna, M.* ; Dominiczak, A.F.* ; Decramer, S.* ; Fliser, D.* ; Frommberger, M. ; Golovko, I.* ; Good, D.M.* ; Herget-Rosenthal, S.* ; Jankowski, J.* ; Julian, B.A.* ; Kellmann, M.* ; Kolch, W.* ; Massy, Z.* ; Novak, J.* ; Rossing, K.* ; Schanstra, J.P.* ; Schiffer, E.* ; Theodorescu, D.* ; Vanholder, R.* ; Weissinger, E.M.* ; Mischak, H.* ; Schmitt-Kopplin, P.

CE-MS analysis of the human urinary proteome for biomarker discovery and disease diagnostics.

Proteomics Clin. Appl. 2, 964-973 (2008)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
Owing to its availability, ease of collection, and correlation with pathophysiology of diseases, urine is an attractive source for clinical proteomics. However, many proteomic studies have had only limited clinical impact, due to factors such as modest numbers of subjects, absence of disease controls, small numbers of defined biomarkers, and diversity of analytical platforms. Therefore, it is difficult to merge biomarkers from different studies into a broadly applicable human urinary proteome database. Ideally, the methodology for defining the biomarkers should combine a reasonable analysis time with high resolution, thereby enabling the profiling of adequate samples and recognition of sufficient features to yield robust diagnostic panels. CE-MS, which was used to analyze urine samples from healthy subjects and patients with various diseases, is a suitable approach for this task. The database of these datasets compiled from the urinary peptides enables the diagnosis, classification, and monitoring of a wide range of diseases. CE-MS exhibits excellent performance for biomarker discovery and allows subsequent biomarker sequencing independent of the separation platform. This approach may elucidate the pathogenesis of many diseases, and better define especially renal and urological disorders at the molecular level.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords CE; Database; MS; Urine
ISSN (print) / ISBN 1862-8346
e-ISSN 1862-8354
Journal Proteomics - Clinical Applications
Quellenangaben Volume: 2, Issue: 7-8, Pages: 964-973 Article Number: , Supplement: ,
Publisher Wiley
Publishing Place Weinheim
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Ecological Chemistry (IOEC)