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Schmitt, S. ; Saathoff, F.* ; Meissner, L.* ; Schropp, E.-M. ; Lichtmannegger, J. ; Schulz, S. ; Eberhagen, C. ; Borchard, S. ; Aichler, M. ; Adamski, J. ; Plesnila, N.* ; Rothenfusser, S.* ; Kroemer, G.* ; Zischka, H.

A semi-automated method for isolating functionally intact mitochondria from cultured cells and tissue biopsies.

Anal. Biochem. 443, 66-74 (2013)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Mitochondrial dysfunctions decisively contribute to the progression of human diseases, implying that functional tests of isolated mitochondria may furnish conclusive information for diagnosis and therapy. Classical mitochondrial isolation methods, however, lack precisely adjustable settings for cell rupture, which is the most critical step in this procedure, and this complicates subsequent analyses. Here, we present an efficient method to isolate functionally active, intact mitochondria from cultured or primary cells and minute tissue samples in a rapid, highly reproducible manner.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Mitochondria ; Cell Culture ; Biopsies ; Balch Homogenizer; Stimulated Glut4 Translocation ; Permeability Transition ; Rat Liver ; Electrophoresis ; Quantitation ; Membrane ; Proteins ; Death
ISSN (print) / ISBN 0003-2697
e-ISSN 1096-0309
Journal Analytical Biochemistry
Quellenangaben Volume: 443, Issue: 1, Pages: 66-74 Article Number: , Supplement: ,
Publisher Elsevier
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Molecular Toxicology and Pharmacology (TOXI)
Molekulare Endokrinologie und Metabolismus (MEM)
Institute of Pathology (PATH)
Research Unit Analytical Pathology (AAP)