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Schmitt, S. ; Saathoff, F.* ; Meissner, L.* ; Schropp, E.-M. ; Lichtmannegger, J. ; Schulz, S. ; Eberhagen, C. ; Borchard, S. ; Aichler, M. ; Adamski, J. ; Plesnila, N.* ; Rothenfusser, S.* ; Kroemer, G.* ; Zischka, H.

A semi-automated method for isolating functionally intact mitochondria from cultured cells and tissue biopsies.

Anal. Biochem. 443, 66-74 (2013)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Mitochondrial dysfunctions decisively contribute to the progression of human diseases, implying that functional tests of isolated mitochondria may furnish conclusive information for diagnosis and therapy. Classical mitochondrial isolation methods, however, lack precisely adjustable settings for cell rupture, which is the most critical step in this procedure, and this complicates subsequent analyses. Here, we present an efficient method to isolate functionally active, intact mitochondria from cultured or primary cells and minute tissue samples in a rapid, highly reproducible manner.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Mitochondria ; Cell Culture ; Biopsies ; Balch Homogenizer; Stimulated Glut4 Translocation ; Permeability Transition ; Rat Liver ; Electrophoresis ; Quantitation ; Membrane ; Proteins ; Death
Language english
Publication Year 2013
HGF-reported in Year 2013
ISSN (print) / ISBN 0003-2697
e-ISSN 1096-0309
Journal Analytical Biochemistry
Quellenangaben Volume: 443, Issue: 1, Pages: 66-74 Article Number: , Supplement: ,
Publisher Elsevier
Reviewing status Peer reviewed
Institute(s) Institute of Molecular Toxicology and Pharmacology (TOX)
Molekulare Endokrinologie und Metabolismus (MEM)
Institute of Pathology (PATH)
Research Unit Analytical Pathology (AAP)
POF-Topic(s) 30203 - Molecular Targets and Therapies
30201 - Metabolic Health
30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
30205 - Bioengineering and Digital Health
Research field(s) Enabling and Novel Technologies
Genetics and Epidemiology
PSP Element(s) G-505200-003
G-505600-001
G-500300-001
G-500390-001
PubMed ID 23969012
DOI 10.1016/j.ab.2013.08.007
WOS ID WOS:000326213900011
Scopus ID 84884599409
Erfassungsdatum 2013-09-05
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