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Steinberger, J.* ; Kontaxis, G.* ; Rancan, C. ; Skern, T.*

Comparison of self-processing of foot-and-mouth disease virus leader proteinase and porcine reproductive and respiratory syndrome virus leader proteinase nsp1α.

Virology 443, 271-277 (2013)

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The foot-and-mouth disease virus leader proteinase (Lb(pro)) cleaves itself off the nascent viral polyprotein. NMR studies on the monomeric variant Lb(pro) L200F provide structural evidence for intramolecular self-processing. N-15-HSQC measurements of Lb(pro) L200F showed specifically shifted backbone signals in the active and substrate binding sites compared to the monomeric variant sLb(pro), lacking six C-terminal residues. This indicates transient intramolecular interactions between the C-terminal extension (CTE) of one molecule and its own active site. Contrastingly, the porcine reproductive and respiratory syndrome virus (PRRSV) leader proteinase nsp1 alpha, with a papain-like fold like Lb(pro), stably binds its own CTE. Parts of the beta-sheet domains but none of the alpha-helical domains of Lb(pro) and nsp1 alpha superimpose; consequently, the alpha-helical domain of nsp1 alpha is oriented differently relative to its beta-sheet domain. This provides a large interaction surface for the CTE with the globular domain, stabilising the intramolecular complex. Consequently, self-processing inactivates nsp1 alpha but not Lb(pro).

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Papain-like Cysteine Proteinase ; Protein Fold ; Active Site ; Polyprotein Processing ; Substrate Binding; Papain-like Enzymes ; Substrate-specificity ; Protease ; Cleavage ; Alignment

ISSN (print) / ISBN 0042-6822

e-ISSN 0042-6822

Journal Virology

Quellenangaben Volume: 443, Issue: 2, Pages: 271-277

Publisher Elsevier

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Research Unit Gene Vector (AGV)