OpenSSL SSL_connect: Connection reset by peer in connection to v2.sherpa.ac.uk:443 PuSH - Publication Server of Helmholtz Zentrum München: DMSO enhances electrospray response, boosting sensitivity of proteomic experiments.

PuSH - Publication Server of Helmholtz Zentrum München

Hahne, H.* ; Pachl, F.* ; Ruprecht, B.* ; Maier, S.K. ; Klaeger, S.* ; Helm, D.* ; Médard, G.* ; Wilm, M.* ; Lemeer, S.* ; Kuster, B.*

DMSO enhances electrospray response, boosting sensitivity of proteomic experiments.

Nat. Methods 10, 989-991 (2013)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
We report that low percentages of dimethylsulfoxide (DMSO) in liquid chromatography solvents lead to a strong enhancement of electrospray ionization of peptides, improving the sensitivity of protein identification in bottom-up proteomics by up to tenfold. The method can be easily implemented on any LC-MS/MS system without modification to hardware or software and at no additional cost.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Mass-spectrometry ; Peptide Identification ; Nanoelectrospray ; Ionization ; Molecules
ISSN (print) / ISBN 1548-7091
e-ISSN 1548-7105
Journal Nature Methods
Quellenangaben Volume: 10, Issue: 10, Pages: 989-991 Article Number: , Supplement: ,
Publisher Nature Publishing Group
Publishing Place New York, NY
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Pathology (PATH)
Research Unit Analytical Pathology (AAP)