PuSH - Publication Server of Helmholtz Zentrum München: Chromosome analysis of the differential radiosensitivity of an Epstein-Barr virus (EBV)-transformed B cell line and B and T lymphocytes from the same blood donor.

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Schmid, E.* ; Roos, H.* ; Sauter, W. ; Rickinger, A. ; Jaehnert, I. ; Eckardt-Schupp, F.

Chromosome analysis of the differential radiosensitivity of an Epstein-Barr virus (EBV)-transformed B cell line and B and T lymphocytes from the same blood donor.

Int. J. Radiat. Biol. 86, 47-55 (2010)

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Open Access Green as soon as Postprint is submitted to ZB.

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PURPOSE: To date, simultaneously performed investigations on the differential radiosensitivity of an Epstein-Barr virus (EBV)-transformed B cell line as well as B and T lymphocytes of human peripheral blood are not available. Thus the aim of the present study was to fill this gap by directly comparing the corresponding dose-response relationships of dicentrics obtained in blood samples from the same donor. MATERIAL AND METHODS: Cell samples of whole blood or low passage cells of an EBV-transformed B cell line were irradiated by 120 kV X-rays in chambers tightly embedded in a polymethylmethacrylate phantom. Chromosome analysis was performed in phytohemagglutinin-stimulated T lymphocytes, in pokeweed mitogen-stimulated B lymphocytes and in the EBV-transformed B cell line. RESULTS: Based on dose-response relationships of dicentrics, different radiosensitivity values relative to T lymphocytes were found from 1.53-1.46 for the EBV-transformed cell line, from 0.76-0.80 for resting B lymphocytes and from 2.36-2.20 for cycling B lymphocytes within the dose range from 0.25-4 Gy. CONCLUSIONS: Owing to these different radiosensitivity values, care has to be taken when dose-response relationships of dicentrics determined in B cell lines are used in biological dosimetry to estimate any dose levels for radiation protection purposes.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords X-rays; Dicentrics; T and B lymphocytes; EBV-transformed cell line

ISSN (print) / ISBN 0955-3002

e-ISSN 1362-3095

Journal International Journal of Radiation Biology

Quellenangaben Volume: 86, Issue: 1, Pages: 47-55

Publisher Informa Healthcare

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Radiation Biology (ISB)
Institute of Epidemiology (EPI)