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Daniel, H.* ; Gholami, A.M.* ; Berry, D.* ; Desmarchelier, C.* ; Hahne, H.* ; Loh, G.* ; Mondot, S.* ; Lepage, P.* ; Rothballer, M. ; Walker, A.* ; Böhm, C.* ; Wenning, M.* ; Wagner, M.* ; Blaut, M.* ; Schmitt-Kopplin, P. ; Kuster, B.* ; Haller, D.* ; Clavel, T.*

High-fat diet alters gut microbiota physiology in mice.

ISME J. 8, 295–308 (2014)
Publ. Version/Full Text DOI PMC
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The intestinal microbiota is known to regulate host energy homeostasis and can be influenced by high-calorie diets. However, changes affecting the ecosystem at the functional level are still not well characterized. We measured shifts in cecal bacterial communities in mice fed a carbohydrate or high-fat (HF) diet for 12 weeks at the level of the following: (i) diversity and taxa distribution by high-throughput 16S ribosomal RNA gene sequencing; (ii) bulk and single-cell chemical composition by Fourier-transform infrared- (FT-IR) and Raman micro-spectroscopy and (iii) metaproteome and metabolome via high-resolution mass spectrometry. High-fat diet caused shifts in the diversity of dominant gut bacteria and altered the proportion of Ruminococcaceae (decrease) and Rikenellaceae (increase). FT-IR spectroscopy revealed that the impact of the diet on cecal chemical fingerprints is greater than the impact of microbiota composition. Diet-driven changes in biochemical fingerprints of members of the Bacteroidales and Lachnospiraceae were also observed at the level of single cells, indicating that there were distinct differences in cellular composition of dominant phylotypes under different diets. Metaproteome and metabolome analyses based on the occurrence of 1760 bacterial proteins and 86 annotated metabolites revealed distinct HF diet-specific profiles. Alteration of hormonal and anti-microbial networks, bile acid and bilirubin metabolism and shifts towards amino acid and simple sugars metabolism were observed. We conclude that a HF diet markedly affects the gut bacterial ecosystem at the functional level.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Diet-induced Obesity ; High-fat Diet ; Intestinal Microbiota ; Metabolomics ; Metaproteome ; Spectroscopy; In-situ Hybridization; Intestinal Microbiota; Induced Obesity; Oligonucleotide Probes; Bacterial-populations; Raman-spectroscopy; Weight-loss; Reveals; Cholesterol; Database
Language english
Publication Year 2014
Prepublished in Year 2013
HGF-reported in Year 2013
ISSN (print) / ISBN 1751-7362
e-ISSN 1751-7370
Journal ISME Journal
Quellenangaben Volume: 8, Issue: 2, Pages: 295–308 Article Number: , Supplement: ,
Publisher Nature Publishing Group
Reviewing status Peer reviewed
Institute(s) Research Unit Microbe-Plant Interactions (AMP)
Research Unit BioGeoChemistry and Analytics (BGC)
POF-Topic(s) 20402 - Sustainable Plant Production
30202 - Environmental Health
Research field(s) Environmental Sciences
PSP Element(s) G-504600-001
G-504800-001
PubMed ID 24030595
DOI 10.1038/ismej.2013.155
WOS ID WOS:000330386500005
Scopus ID 84893013094
Erfassungsdatum 2013-09-26
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