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Rezeptor-mediierter Gentransfer in Plattenepithelkarzinome des Kopf-Hals-Bereiches.
Receptor mediated gene transfer in squamous epithelial carcinoma of the head-neck area.
Laryngol. Rhinol. Otol. 76, 411-414 (1997)
BACKGROUND: The "Adeno-Virus-Enhanced-Receptor-Mediated" (AVET)-System uses the receptor-mediated endocytosis route to introduce DNA into mammalian cells. By the help of a replication defective adenovirus with endosomolytic activity the coentry of receptor-bound DNA particles is faciliated. The transport to the nucleus followed by gene expression is multifold enhanced. METHODS: Two different permanent cell lines from head and neck squamous cell cancer were transfected with the reportergene for luciferase with either AVET or different liposoms in vitro. RESULTS: In comparison it becomes obvious, that AVET shows a multifold higher transfection rate in vitro than the liposomal formulations applied. CONCLUSIONS: AVET could be used for efficient transfection of epithelial cells, that are fairly reluctant to DNA transfer. Further trials in the mouse model must prove a possible application in vivo.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
0302-9379
e-ISSN
1438-8685
Journal
Laryngo-Rhino-Otologie
Quellenangaben
Volume: 76,
Issue: 7,
Pages: 411-414
Publisher
Thieme
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Research Unit Gene Vector (AGV)