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Dallmann, A. ; Simon, B.* ; Duszczyk, M.M. ; Kooshapur, H. ; Pardi, A.* ; Bermel, W.* ; Sattler, M.

Efficient detection of hydrogen bonds in dynamic regions of RNA by sensitivity-optimized NMR pulse sequences.

Angew. Chem.-Int. Edit. 52, 10487-10490 (2013)

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Open Access Green as soon as Postprint is submitted to ZB.

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Improved Sensitivity: Efficient NMR experiments are presented for determining the secondary structure in large and dynamic RNAs using J-couplings across hydrogen bonds. The experiments provide up to eight-fold improved sensitivity and thus enable detection of base pairs in dynamic regions even in large RNAs.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords NMR spectroscopy; RNA dynamics; RNA secondary structure; base pairs; hydrogen bonds; Crick Base-pairs ; Scalar Couplings ; Secondary Structure ; Nucleic-acids ; Structure Prediction ; Crystal-structure ; A-repeat ; Tetraloop ; Proteins ; Motions

ISSN (print) / ISBN 1433-7851

e-ISSN 1521-3773

Journal Angewandte Chemie - Internationale Edition

Quellenangaben Volume: 52, Issue: 40, Pages: 10487-10490

Publisher Wiley

Publishing Place Weinheim

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Structural Biology (STB)