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Sieper, T. ; Forczek, S.* ; Matucha, M.* ; Krämer, P. ; Hartmann, A. ; Schröder, P.

N-acyl-homoserine lactone uptake and systemic transport in barley rest upon active parts of the plant.

New Phytol. 201, 545-555 (2014)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Bacteria communicate with each other in a population density-dependent process known as quorum sensing. N-acyl-homoserine lactones (HSLs) are the autoinducers of Gram-negative bacteria and the best-studied quorum sensing signals so far. HSLs induce various responses in plants, including systemic resistance and root development. Here, we used different methods, including tritium labelling, sensor strain assays and monoclonal antibodies (mAbs), to analyse the uptake and translocation of C8- and C10- homoserine lactones into barley (Hordeum vulgare cv Barke). Both HSLs were already systemically transported into the shoot at 2 h after application. HSL uptake could be inhibited by orthovanadate, demonstrating that ABC transporters are involved in the uptake. Root transport occurs predominantly via the central cylinder, which was shown by transport inhibition via KCl application and autoradiography of root cross-sections. Furthermore, a newly established detection method with mAbs allowed the first detection of a systemic transport of long-chain HSLs in plants. The coupled use of different HSL detection methods demonstrated that the uptake and transport of HSLs into barley does not occur passively, but relies, at least partially, on active processes in the plant.
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Publication type Article: Journal article
Document type Scientific Article
Keywords N-acyl-homoserine lactones (HSLs); barley (Hordeum vulgare); monoclonal antibodies; quorum sensing; signalling; Serratia-liquefaciens; Arabidopsis-thaliana; Root Development; Bacteria; Rhizosphere; Signals; Communication; Degradation; Pseudomonas; Resistance
ISSN (print) / ISBN 0028-646X
e-ISSN 1469-8137
Journal New Phytologist
Quellenangaben Volume: 201, Issue: 2, Pages: 545-555 Article Number: , Supplement: ,
Publisher Wiley
Reviewing status Peer reviewed
Institute(s) Research Unit Microbe-Plant Interactions (AMP)