PuSH - Publication Server of Helmholtz Zentrum München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Mascher, M.* ; Muehlbauer, G.J.* ; Rokhsar, D.S.* ; Chapman, J.* ; Schmutz, J.* ; Barry, K.* ; Muñoz-Amatriaín, M.* ; Close, T.J.* ; Wise, R.P.* ; Schulman, A.H.* ; Himmelbach, A.* ; Mayer, K.F.X. ; Scholz, U.* ; Poland, J.A.* ; Stein, N.* ; Waugh, R.*

Anchoring and ordering NGS contig assemblies by population sequencing (POPSEQ).

Plant J. 76, 718-727 (2013)
Publ. Version/Full Text Volltext DOI PMC
Closed
Open Access Green as soon as Postprint is submitted to ZB.
Next-generation whole-genome shotgun assemblies of complex genomes are highly useful, but fail to link nearby sequence contigs with each other or provide a linear order of contigs along individual chromosomes. Here, we introduce a strategy based on sequencing progeny of a segregating population that allows de novo production of a genetically anchored linear assembly of the gene space of an organism. We demonstrate the power of the approach by reconstructing the chromosomal organization of the gene space of barley, a large, complex and highly repetitive 5.1 Gb genome. We evaluate the robustness of the new assembly by comparison to a recently released physical and genetic framework of the barley genome, and to various genetically ordered sequence-based genotypic datasets. The method is independent of the need for any prior sequence resources, and will enable rapid and cost-efficient establishment of powerful genomic information for many species.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Scopus
Cited By
Altmetric
6.582
1.650
192
206
Tags
Annotations
Special Publikation
Hide on homepage

Edit extra information
Edit own tags
Private
Edit own annotation
Private
Hide on publication lists
on hompage
Mark as special
publikation
Publication type Article: Journal article
Document type Scientific Article
Keywords Next-generation Sequencing ; Genome Assembly ; Genetic Mapping ; Barley ; Hordeum Vulgare ; Population Sequencing ; Technical Advance; Maize Genome ; Draft Genome ; Wheat ; Map ; Association
Language english
Publication Year 2013
HGF-reported in Year 2013
ISSN (print) / ISBN 0960-7412
e-ISSN 1365-313X
Journal Plant Journal, The
Quellenangaben Volume: 76, Issue: 4, Pages: 718-727 Article Number: , Supplement: ,
Publisher Wiley
Reviewing status Peer reviewed
Institute(s) Institute of Bioinformatics and Systems Biology (IBIS)
POF-Topic(s) 20402 - Sustainable Plant Production
Research field(s) Enabling and Novel Technologies
PSP Element(s) G-503790-001
PubMed ID 23998490
DOI 10.1111/tpj.12319
WOS ID WOS:000326138100016
Scopus ID 84886793453
Erfassungsdatum 2013-10-25
[➜Report correction]