OpenSSL SSL_connect: Connection reset by peer in connection to v2.sherpa.ac.uk:443 PuSH - Publication Server of Helmholtz Zentrum München: Improved mass spectrometric identification of gel-separated hydrophobic membrane proteins after sodium dodecyl sulfate removal by ion-pair extraction.

PuSH - Publication Server of Helmholtz Zentrum München

Zischka, H. ; Gloeckner, C.J. ; Klein, C.* ; Willmann, S. ; Swiatek-de Lange, M. ; Ueffing, M.

Improved mass spectrometric identification of gel-separated hydrophobic membrane proteins after sodium dodecyl sulfate removal by ion-pair extraction.

Proteomics 4, 3776-3782 (2004)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Separation and identification of hydrophobic membrane proteins is a major challenge in proteomics. Identification of such sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)-separated proteins by peptide mass fingerprinting (PMF) via matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) is frequently hampered by the insufficient amount of peptides being generated and their low signal intensity. Using the seven helical transmembrane-spanning proton pump bacteriorhodopsin as model protein, we demonstrate here that SDS removal from hydrophobic proteins by ion-pair extraction prior to in-gel tryptic proteolysis leads to a tenfold higher sensitivity in mass spectrometric identification via PMF, with respect to initial protein load on SDS-PAGE. Furthermore, parallel sequencing of the generated peptides by electrospray ionization-mass spectrometry (ESI-MS) and tandem mass spectrometry (MS/MS) was possible without further sample cleanup. We also show identification of other membrane proteins by this protocol, as proof of general applicability.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords bacteriorhodospin; matrix-assisted laser desorption; ionization-time of flight; membrane proteomics; POLYACRYLAMIDE-GEL; SEQUENCE DATABASES; PEPTIDE; BACTERIORHODOPSIN; RHODOPSIN; FRAGMENTS; SAMPLES; LEVEL
ISSN (print) / ISBN 1615-9853
e-ISSN 1615-9861
Journal Proteomics
Quellenangaben Volume: 4, Issue: 12, Pages: 3776-3782 Article Number: , Supplement: ,
Publisher Wiley
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Human Genetics (IHG)