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Open Access Green as soon as Postprint is submitted to ZB.
Development of a capillary isoelectric focusing immunoassay to measure DJ-1 isoforms in biological samples.
Anal. Biochem. 443, 197-204 (2013)
We report on the development of a novel assay protocol for the separation and detection of charge isoforms of DJ-1 in biological samples by automated capillary isoelectric focusing followed by immunological detection. DJ-1 (PARK7) is considered as a biomarker candidate for Parkinson's disease and may potentially support the differentiation of clinical subtypes of the disease. The new method allows for separation and subsequent relative quantitative comparison of different isoforms of DJ-1 in biological samples. The assay was successfully applied to the analysis of DJ-1 isoform patterns in brains from mice subjected to normal or high-fat diet and revealed statistically significant group differences. Furthermore, in a pooled and concentrated sample of human cerebrospinal fluid that was depleted of albumin and immunoglobulin G, four different charge variants of DJ-1 could be detected. Taken together, the capillary isoelectric focusing immunoassay for DJ-1 represents a promising tool that may ultimately serve in clinical biomarker studies.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Biomarker; CSF; DJ-1; Immunoassay; Isoelectric focusing; PARK7; Parkinsons-disease ; 2-dimensional Electrophoresis ; Cerebrospinal-fluid ; Biomarkers ; Cells
ISSN (print) / ISBN
0003-2697
e-ISSN
1096-0309
Journal
Analytical Biochemistry
Quellenangaben
Volume: 443,
Issue: 2,
Pages: 197-204
Publisher
Elsevier
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Developmental Genetics (IDG)