Clues to quality of journals
Open Access Policy of Helmholtz Association 2016
CC Licencences
Publication Metrics
Home
Deutsch
New EVA Application
Advanced Search
Browse by ...
Publication overview
Statistics (last 5 years)
OA Publications
Submit Publication
Import publication from...
Report missing publication
Contact persons
Help
Text
Endnote (RIS)
BibTeX
DOI
PMC
 |
|
Open Access Green as soon as Postprint is submitted to ZB.
Development of a capillary isoelectric focusing immunoassay to measure DJ-1 isoforms in biological samples.
Anal. Biochem. 443, 197-204 (2013)
We report on the development of a novel assay protocol for the separation and detection of charge isoforms of DJ-1 in biological samples by automated capillary isoelectric focusing followed by immunological detection. DJ-1 (PARK7) is considered as a biomarker candidate for Parkinson's disease and may potentially support the differentiation of clinical subtypes of the disease. The new method allows for separation and subsequent relative quantitative comparison of different isoforms of DJ-1 in biological samples. The assay was successfully applied to the analysis of DJ-1 isoform patterns in brains from mice subjected to normal or high-fat diet and revealed statistically significant group differences. Furthermore, in a pooled and concentrated sample of human cerebrospinal fluid that was depleted of albumin and immunoglobulin G, four different charge variants of DJ-1 could be detected. Taken together, the capillary isoelectric focusing immunoassay for DJ-1 represents a promising tool that may ultimately serve in clinical biomarker studies.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Times Cited
Scopus
Cited By
Cited By
Altmetric
2.582
0.942
6
7
Annotations
Special Publikation
Hide on homepage
Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Biomarker; CSF; DJ-1; Immunoassay; Isoelectric focusing; PARK7; Parkinsons-disease ; 2-dimensional Electrophoresis ; Cerebrospinal-fluid ; Biomarkers ; Cells
Language
english
Publication Year
2013
HGF-reported in Year
2013
ISSN (print) / ISBN
0003-2697
e-ISSN
1096-0309
Journal
Analytical Biochemistry
Quellenangaben
Volume: 443,
Issue: 2,
Pages: 197-204
Publisher
Elsevier
Reviewing status
Peer reviewed
Institute(s)
Institute of Developmental Genetics (IDG)
POF-Topic(s)
30204 - Cell Programming and Repair
30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
Research field(s)
Genetics and Epidemiology
PSP Element(s)
G-500500-001
G-500500-003
G-500500-007
G-500500-003
G-500500-007
PubMed ID
24055619
WOS ID
WOS:000327279700013
Scopus ID
84885796697
Erfassungsdatum
2013-11-06