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Culturing and transfecting zebrafish PAC2 fibroblast cells.
Cold Spring Harb. Protoc. 4, 1-7 (2009)
The use of zebrafish (Danio rerio) as a model organism has seen rapid growth in recent years. Numerous new techniques have emerged for zebrafish, and many protocols used in other model organisms have been adapted to fit the special needs of this species. Zebrafish cell lines, however, have not been studied to a great extent, and their applications remain limited. The PAC2 fibroblast line, isolated from 24-h post-fertilization zebrafish embryos, is one of the few available lines. Here, we provide a basic set of methods for maintenance and handling of PAC2 cells, as well as general procedures for transfection, immunocytochemistry, and protein extraction.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
1940-3402
e-ISSN
1559-6095
Journal
Cold Spring Harbor Protocols
Quellenangaben
Volume: 4,
Issue: 6,
Pages: 1-7
Publisher
Cold Spring Harbor Laboratory Press
Reviewing status
Peer reviewed
Institute(s)
Institute of Developmental Genetics (IDG)