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Panda, S. ; Wefers, B. ; Ortiz, O. ; Floß, T. ; Schmid, B.* ; Haass, C.* ; Wurst, W. ; Kühn, R.

Highly efficient targeted mutagenesis in mice using TALENs.

Genetics 195, 703-713 (2013)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Targeted mouse mutants are instrumental for the analysis of gene function in health and disease. We recently provided proof-of-principle for the fast-track mutagenesis of the mouse genome, using transcription activator-like effector nucleases (TALENs) in one-cell embryos. Here we report a routine procedure for the efficient production of disease-related knockin and knockout mutants, using improved TALEN mRNAs that include a plasmid-coded poly(A) tail (TALEN-95A), circumventing the problematic in vitro polyadenylation step. To knock out the C9orf72 gene as a model of frontotemporal lobar degeneration, TALEN-95A mutagenesis induced sequence deletions in 41% of pups derived from microinjected embryos. Using TALENs together with mutagenic oligodeoxynucleotides, we introduced amyotrophic lateral sclerosis patient-derived missense mutations in the fused in sarcoma (Fus) gene at a rate of 6.8%. For the simple identification of TALEN-induced mutants and their progeny we validate high-resolution melt analysis (HRMA) of PCR products as a sensitive and universal genotyping tool. Furthermore, HRMA of off-target sites in mutant founder mice revealed no evidence for undesired TALEN-mediated processing of related genomic sequences. The combination of TALEN-95A mRNAs for enhanced mutagenesis and of HRMA for simplified genotyping enables the accelerated, routine production of new mouse models for the study of genetic disease mechanisms.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Talens ; Disease Model ; One-cell Embryo ; Mouse Mutant ; Nuclease ; Fus ; C9orf72; Zinc-finger Nucleases; Amyotrophic-lateral-sclerosis; Homologous Recombination; Embryo Microinjection; Hexanucleotide Repeat; Mammalian-cells; Gene; C9orf72; Genome; Mouse
ISSN (print) / ISBN 0016-6731
e-ISSN 0016-6731
Journal Genetics
Quellenangaben Volume: 195, Issue: 3, Pages: 703-713 Article Number: , Supplement: ,
Publisher Genetics Society of America
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Developmental Genetics (IDG)