PuSH - Publication Server of Helmholtz Zentrum München: Two herbivore-induced cytochrome P450 enzymes CYP79D6 and CYP79D7 catalyze the formation of volatile aldoximes involved in poplar defense.

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Irmisch, S.* ; Andrea, C.M.* ; Boeckler, G.A.* ; Schmidt, A.* ; Reichelt, M.* ; Schneider, B.* ; Block, K. ; Schnitzler, J.-P. ; Gershenzon, J.* ; Unsicker, S.B.* ; Köllner, T.G.*

Two herbivore-induced cytochrome P450 enzymes CYP79D6 and CYP79D7 catalyze the formation of volatile aldoximes involved in poplar defense.

Plant Cell 25, 4737-4754 (2013)

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Open Access Green as soon as Postprint is submitted to ZB.

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Aldoximes are known as floral and vegetative plant volatiles but also as biosynthetic intermediates for other plant defense compounds. While the cytochrome P450 monooxygenases (CYP) from the CYP79 family forming aldoximes as biosynthetic intermediates have been intensively studied, little is known about the enzymology of volatile aldoxime formation. We characterized two P450 enzymes, CYP79D6v3 and CYP79D7v2, which are involved in herbivore-induced aldoxime formation in western balsam poplar (Populus trichocarpa). Heterologous expression in Saccharomyces cerevisiae revealed that both enzymes produce a mixture of different aldoximes. Knockdown lines of CYP79D6/7 in gray poplar (Populus × canescens) exhibited a decreased emission of aldoximes, nitriles, and alcohols, emphasizing that the CYP79s catalyze the first step in the formation of a complex volatile blend. Aldoxime emission was found to be restricted to herbivore-damaged leaves and is closely correlated with CYP79D6 and CYP79D7 gene expression. The semi-volatile phenylacetaldoxime decreased survival and weight gain of gypsy moth (Lymantria dispar) caterpillars, suggesting that aldoximes may be involved in direct defense. The wide distribution of volatile aldoximes throughout the plant kingdom and the presence of CYP79 genes in all sequenced genomes of angiosperms suggest that volatile formation mediated by CYP79s is a general phenomenon in the plant kingdom.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Caterpillars Malacosoma-disstria; Cyanogenic Glucoside Dhurrin; Manihot-esculenta Crantz; Trichocarpa X Deltoides; Populus-trichocarpa; Lotus-japonicus; Plant Defense; Insect Herbivores; Escherichia-coli; Hybrid Poplar

ISSN (print) / ISBN 1040-4651

e-ISSN 1532-298X

Journal The Plant cell

Quellenangaben Volume: 25, Issue: 11, Pages: 4737-4754

Publisher American Society of Plant Biologists (ASPB)

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Research Unit Environmental Simulation (BIOP-EUS)