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Open Access Green as soon as Postprint is submitted to ZB.
Energy-dependent uptake of benzo[a]pyrene and its cytoskeleton-dependent intracellular transport by the telluric fungus Fusarium solani.
Environ. Sci. Pollut. Res. 21, 3515-3523 (2014)
In screening indigenous soil filamentous fungi for polycyclic aromatic hydrocarbons (PAHs) degradation, an isolate of the Fusarium solani was found to incorporate benzo[a]pyrene (BaP) into fungal hyphae before degradation and mineralization. The mechanisms involved in BaP uptake and intracellular transport remain unresolved. To address this, the incorporation of two PAHs, BaP, and phenanthrene (PHE) were studied in this fungus. The fungus incorporated more BaP into cells than PHE, despite the 400-fold higher aqueous solubility of PHE compared with BaP, indicating that PAH incorporation is not based on a simple diffusion mechanism. To identify the mechanism of BaP incorporation and transport, microscopic studies were undertaken with the fluorescence probes Congo Red, BODIPY®493/503, and FM®4-64, targeting different cell compartments respectively fungal cell walls, lipids, and endocytosis. The metabolic inhibitor sodium azide at 100 mM totally blocked BaP incorporation into fungal cells indicating an energy-requirement for PAH uptake into the mycelium. Cytochalasins also inhibited BaP uptake by the fungus and probably its intracellular transport into fungal hyphae. The perfect co-localization of BaP and BODIPY reveals that lipid bodies constitute the intracellular storage sites of BaP in F. solani. Our results demonstrate an energy-dependent uptake of BaP and its cytoskeleton-dependent intracellular transport by F. solani.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Active transport; Benzo[a]pyrene; Confocal microscopy; Endocytosis; Fusarium solani; Polycyclic Aromatic-hydrocarbons; Cladosporium-resinae; Aspergillus-nidulans; Oleaginous Fungus; Radical Cations; Lipid Bodies; Rot Fungus; Congo Red; Endocytosis; Activation
ISSN (print) / ISBN
0944-1344
e-ISSN
1614-7499
Quellenangaben
Volume: 21,
Issue: 5,
Pages: 3515-3523
Publisher
Springer
Publishing Place
Heidelberg
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Soil Ecology (IBOE)