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Szymczak, W. ; Rozman, J. ; Höllriegl, V. ; Kistler, M. ; Keller, S. ; Peters, D. ; Kneipp, M. ; Schulz, H. ; Hoeschen, C. ; Klingenspor, M.* ; Hrabě de Angelis, M.

Online breath gas analysis in unrestrained mice by hs-PTR-MS.

Mamm. Genome 25, 129-140 (2014)
Postprint DOI PMC
Open Access Green
The phenotyping of genetic mouse models for human disorders may greatly benefit from breath gas analysis as a noninvasive tool to identify metabolic alterations in mice. Phenotyping screens such as the German Mouse Clinic demand investigations in unrestrained mice. Therefore, we adapted a breath screen in which exhaled volatile organic compounds (VOCs) were online monitored by proton transfer reaction mass spectrometry (hs-PTR-MS). The source strength of VOCs was derived from the dynamics in the accumulation profile of exhaled VOCs of a single mouse in a respirometry chamber. A careful survey of the accumulation revealed alterations in the source strength due to confounders, e.g., urine and feces. Moreover changes in the source strength of humidity were triggered by changes in locomotor behavior as mice showed a typical behavioral pattern from activity to settling down in the course of subsequent accumulation profiles. We demonstrated that metabolic changes caused by a dietary intervention, e.g., after feeding a high-fat diet (HFD) a sample of 14 male mice, still resulted in a statistically significant shift in the source strength of exhaled VOCs. Applying a normalization which was derived from the distribution of the source strength of humidity and accounted for varying locomotor behaviors improved the shift. Hence, breath gas analysis may provide a noninvasive, fast access to monitor the metabolic adaptation of a mouse to alterations in energy balance due to overfeeding or fasting and dietary macronutrient composition as well as a high potential for systemic phenotyping of mouse mutants, intervention studies, and drug testing in mice.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Volatile Organic-compounds; Mass-spectrometry; Gut Microbiota; Lung-function; Mouse; Diet; Phenotype; Ethane; Cancer; Index
Language english
Publication Year 2014
Prepublished in Year 2013
HGF-reported in Year 2013
ISSN (print) / ISBN 0938-8990
e-ISSN 1432-1777
Journal Mammalian Genome
Quellenangaben Volume: 25, Issue: 3-4, Pages: 129-140 Article Number: , Supplement: ,
Publisher Springer
Publishing Place New York
Reviewing status Peer reviewed
Institute(s) Research Unit Medical Radiation Physics and Diagnostics (AMSD)
Institute of Experimental Genetics (IEG)
Institute of Epidemiology (EPI)
POF-Topic(s) 30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
30201 - Metabolic Health
30503 - Chronic Diseases of the Lung and Allergies
90000 - German Center for Diabetes Research
Research field(s) Radiation Sciences
Genetics and Epidemiology
PSP Element(s) G-503600-001
G-500600-003
G-500600-001
G-503900-003
G-501900-063
G-501900-066
PubMed ID 24275888
DOI 10.1007/s00335-013-9493-8
WOS ID WOS:000333184000004
Scopus ID 84897112190
Erfassungsdatum 2013-11-27
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