OpenSSL SSL_connect: Connection reset by peer in connection to v2.sherpa.ac.uk:443 PuSH - Publication Server of Helmholtz Zentrum München: Microscopic visualization of metabotropic glutamate receptors on the surface of living cells using bifunctional magnetic resonance imaging probes.

PuSH - Publication Server of Helmholtz Zentrum München

Mishra, A. ; Mishra, R.* ; Gottschalk, S. ; Pal, R.* ; Sim, N.* ; Engelmann, J.* ; Goldberg, M.* ; Parker, D.*

Microscopic visualization of metabotropic glutamate receptors on the surface of living cells using bifunctional magnetic resonance imaging probes.

ACS Chem. Neurosci. 5, 128-137 (2014)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
A series of bimodal metabotropic glutamate-receptor targeted MRI contrast agents has been developed and evaluated, based on established competitive metabotropic Glu receptor subtype 5 (mGluR5) antagonists. In order to directly visualize mGluR5 binding of these agents on the surface of live astrocytes, variations in the core structure were made. A set of gadolinium conjugates containing either a cyanine dye or a fluorescein moiety was accordingly prepared, to allow visualization by optical microscopy in cellulo. In each case, surface receptor binding was compromised and cell internalization observed. Another approach, examining the location of a terbium analogue via sensitized emission, also exhibited nonspecific cell uptake in neuronal cell line models. Finally, biotin derivatives of two lead compounds were prepared, and the specificity of binding to the mGluR5 cell surface receptors was demonstrated with the aid of their fluorescently labeled avidin conjugates, using both total internal reflection fluorescence (TIRF) and confocal microscopy.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Imaging Agents ; Lanthanides ; Mglur5 ; Microscopy ; Mri
e-ISSN 1948-7193
Journal ACS Chemical Neuroscience
Quellenangaben Volume: 5, Issue: 2, Pages: 128-137 Article Number: , Supplement: ,
Publisher American Chemical Society (ACS)
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Biological and Medical Imaging (IBMI)