PuSH - Publication Server of Helmholtz Zentrum München

Chronakova, A.* ; Radl, V.* ; Cuhel, J.* ; Simek, M.* ; Elhottova, D.* ; Engel, M.* ; Schloter, M.

Overwintering management on upland pasture causes shifts in an abundance of denitrifying microbial communities, their activity and N₂O-reducing ability.

Soil Biol. Biochem. 41, 1132-1138 (2009)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Pasture soils used for cattle overwintering may represent significant sources of N2O emissions from soils. Therefore, the long-term effect of cattle overwintering on the abundance and activity of a denitrifying community was explored. The study was performed at a cattle overwintering area in South Bohemia (Czech Republic), where three sites differing in the degree of animal impact were selected: severely impacted (SI) and moderately impacted (MI), as well as a control site with no impact (NI). N2O flux measurement and soil sampling were performed in spring and fall of 2005. The activity was measured in terms of potential denitrification activity. Bacterial nirK, nirS and nosZ genes were used as functional markers of the denitrifying communities; abundance was analyzed using a real-time PCR assay. Surprisingly, in situ N2O emissions were the highest in spring at MI and significantly differed from those at SI and NI, while in autumn, rates of emissions generally decreased. In contrast potential denitrification rates were highest at SI, followed by MI. and the lowest at NI. An overall significant shift in N2O/N-2 molar ratio was shown in cattle impacted sites. The highest abundance of all genes measured at both sampling times was found at site SI, whereas at site MI increased numbers were observed only in spring. Our results indicate a strong influence of cattle on the abundance as well as the activity of microbes involved in denitrification.
Altmetric
Additional Metrics?
[➜Log in]
Edit extra informations Login
Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Nitrous oxide; qPCR; nirS; nirK; nosZ; DEA; Grassland soils; nitrous-oxide emissions; reductase genes nirk; functional genes; ribosomal-rna; nosz genes; soil; denitrification; diversity; bacteria; nitrate
ISSN (print) / ISBN 0038-0717
e-ISSN 1879-3428
Journal Soil Biology & Biochemistry
Quellenangaben Volume: 41, Issue: 6, Pages: 1132-1138 Article Number: , Supplement: ,
Publisher Elsevier
Publishing Place Amsterdam
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Soil Ecology (IBOE)