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Open Access Gold as soon as Publ. Version/Full Text is submitted to ZB.
Early dynamic fate changes in haemogenic endothelium characterized at the single-cell level.
Nat. Commun. 4:2924 (2013)
Haematopoietic stem cells (HSCs) are the founding cells of the adult haematopoietic system, born during ontogeny from a specialized subset of endothelium, the haemogenic endothelium (HE) via an endothelial-to-haematopoietic transition (EHT). Although recently imaged in real time, the underlying mechanism of EHT is still poorly understood. We have generated a Runx1 +23 enhancer-reporter transgenic mouse (23GFP) for the prospective isolation of HE throughout embryonic development. Here we perform functional analysis of over 1,800 and transcriptional analysis of 268 single 23GFP(+) HE cells to explore the onset of EHT at the single-cell level. We show that initiation of the haematopoietic programme occurs in cells still embedded in the endothelial layer, and is accompanied by a previously unrecognized early loss of endothelial potential before HSCs emerge. Our data therefore provide important insights on the timeline of early haematopoietic commitment.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Hematopoietic Stem-cells; Definitive Hematopoiesis; Mouse Embryo; Aortic Endothelium; Runx1 Expression; Fetal Liver; Agm Region; Lymphohematopoietic Cells; Differentiation Cultures; Blood Progenitors
ISSN (print) / ISBN
2041-1723
e-ISSN
2041-1723
Journal
Nature Communications
Quellenangaben
Volume: 4,
Article Number: 2924
Publisher
Nature Publishing Group
Publishing Place
London
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Research Unit Stem Cell Dynamics (SCD)