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Chemically linked phage idiotype vaccination in the murine B cell lymphoma 1 model.
J. Transl. Med. 11:267 (2013)
Background: B cell malignancies are characterized by clonal expansion of B cells expressing tumor-specific idiotypes on their surface. These idiotypes are ideal target antigens for an individualized immunotherapy. However, previous idiotype vaccines mostly lacked efficiency due to a low immunogenicity of the idiotype. The objective of the present study was the determination of the feasibility, safety and immunogenicity of a novel chemically linked phage idiotype vaccine. Methods: In the murine B cell lymphoma 1 model, tumor idiotypes were chemically linked to phage particles used as immunological carriers. For comparison, the idiotype was genetically expressed on the major phage coat protein g8 or linked to keyhole limpet hemocynanin. After intradermal immunizations with idiotype vaccines, tolerability and humoral immune responses were assessed. Results: Feasibility and tolerability of the chemically linked phage idiotype vaccine was demonstrated. Vaccination with B cell lymphoma 1 idiotype expressing phage resulted in a significant survival benefit in the murine B cell lymphoma 1 protection model (60.2 +/- 23.8 days vs. 41.8 +/- 1.6 days and 39.8 +/- 3.8 days after vaccination with wild type phage or phosphate buffered saline, respectively). Superior immunogenicity of the chemically linked phage idiotype vaccine compared to the genetically engineered phage idiotype and keyhole limpet hemocynanin-coupled idiotype vaccine was demonstrated by significantly higher B cell lymphoma 1 idiotype-specific IgG levels after vaccination with chemically linked phage idiotype. Conclusion: We present a novel, simple, time-and cost-efficient phage idiotype vaccination strategy, which represents a safe and feasible therapy and may produce a superior immune response compared to previously employed idiotype vaccination strategies.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Phage Idiotype Vaccination ; B Cell Lymphoma ; Murine Bcl1 Lymphoma Model ; Klh; Colony-stimulating Factor; Multiple-myeloma; Antibody-responses; Filamentous Phage; Dendritic Cells; Tumor; Antigen; Peptide; Bcl1; Epitopes
ISSN (print) / ISBN
1479-5876
e-ISSN
1479-5876
Quellenangaben
Volume: 11,
Issue: 1,
Article Number: 267
Publisher
BioMed Central
Non-patent literature
Publications
Reviewing status
Peer reviewed